Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19682 | 3' | -50.2 | NC_004686.1 | + | 17840 | 0.66 | 0.942398 |
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Target: 5'- ---aUGAGCCAugGUCACucaCCCCCAc- -3' miRNA: 3'- guaaGCUUGGU--UAGUGua-GGGGGUca -5' |
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| 19682 | 3' | -50.2 | NC_004686.1 | + | 6915 | 0.66 | 0.942398 |
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Target: 5'- gGUUCGGACCAAgCGCGUaCUUCAGc -3' miRNA: 3'- gUAAGCUUGGUUaGUGUAgGGGGUCa -5' |
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| 19682 | 3' | -50.2 | NC_004686.1 | + | 52089 | 0.66 | 0.931579 |
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Target: 5'- --aUCGAuaaacGCCAAUgGCG-CCCCCAa- -3' miRNA: 3'- guaAGCU-----UGGUUAgUGUaGGGGGUca -5' |
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| 19682 | 3' | -50.2 | NC_004686.1 | + | 22336 | 0.67 | 0.913114 |
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Target: 5'- --cUCGcGGCCGGccuUCGCGUCaCCCCGGc -3' miRNA: 3'- guaAGC-UUGGUU---AGUGUAG-GGGGUCa -5' |
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| 19682 | 3' | -50.2 | NC_004686.1 | + | 1423 | 0.67 | 0.904979 |
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Target: 5'- uGUUCGGgaccgugACCGAUCACAgUCCauuugauCCCGGUu -3' miRNA: 3'- gUAAGCU-------UGGUUAGUGU-AGG-------GGGUCA- -5' |
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| 19682 | 3' | -50.2 | NC_004686.1 | + | 5086 | 0.67 | 0.884372 |
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Target: 5'- aCGUUCGcuCCcGUCACcgccAUCCCCCGc- -3' miRNA: 3'- -GUAAGCuuGGuUAGUG----UAGGGGGUca -5' |
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| 19682 | 3' | -50.2 | NC_004686.1 | + | 6574 | 0.69 | 0.813059 |
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Target: 5'- gGUUCGAucaaacgaggggcGCCAGUCGCAgCCCCUu-- -3' miRNA: 3'- gUAAGCU-------------UGGUUAGUGUaGGGGGuca -5' |
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| 19682 | 3' | -50.2 | NC_004686.1 | + | 34459 | 0.7 | 0.780874 |
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Target: 5'- gGUUCGAACgCAGccuUCACGgcgaccgacgggguUCCUCCAGUg -3' miRNA: 3'- gUAAGCUUG-GUU---AGUGU--------------AGGGGGUCA- -5' |
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| 19682 | 3' | -50.2 | NC_004686.1 | + | 37770 | 0.73 | 0.617519 |
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Target: 5'- gCAUUCGAAUacggauaauUCGCGUCCCCCGa- -3' miRNA: 3'- -GUAAGCUUGguu------AGUGUAGGGGGUca -5' |
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| 19682 | 3' | -50.2 | NC_004686.1 | + | 29912 | 1.08 | 0.003783 |
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Target: 5'- uCAUUCGAACCAAUCACAUCCCCCAGUg -3' miRNA: 3'- -GUAAGCUUGGUUAGUGUAGGGGGUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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