Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19683 | 3' | -53.6 | NC_004686.1 | + | 3895 | 0.67 | 0.763147 |
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Target: 5'- uGGCAGU-UCUGGCAUUcgcagcCGAGGGGGc -3' miRNA: 3'- uCUGUCGaAGGUCGUAGu-----GCUCUCCC- -5' |
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| 19683 | 3' | -53.6 | NC_004686.1 | + | 11127 | 0.67 | 0.763147 |
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Target: 5'- gGGACGuGCggCCAGUGUUugGcGAGGa -3' miRNA: 3'- -UCUGU-CGaaGGUCGUAGugCuCUCCc -5' |
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| 19683 | 3' | -53.6 | NC_004686.1 | + | 412 | 0.67 | 0.720835 |
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Target: 5'- cGGGCGGUcaaCCGGCggGUC-CGAGAGGa -3' miRNA: 3'- -UCUGUCGaa-GGUCG--UAGuGCUCUCCc -5' |
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| 19683 | 3' | -53.6 | NC_004686.1 | + | 13644 | 0.68 | 0.688112 |
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Target: 5'- --cCAGCUguUCCAGCGUCAaaucCGGGAcacggcGGGg -3' miRNA: 3'- ucuGUCGA--AGGUCGUAGU----GCUCU------CCC- -5' |
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| 19683 | 3' | -53.6 | NC_004686.1 | + | 17452 | 0.69 | 0.654893 |
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Target: 5'- uGACGGUgUgCAGCcgCGCGAGGuguGGGa -3' miRNA: 3'- uCUGUCGaAgGUCGuaGUGCUCU---CCC- -5' |
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| 19683 | 3' | -53.6 | NC_004686.1 | + | 30058 | 1.11 | 0.001033 |
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Target: 5'- cAGACAGCUUCCAGCAUCACGAGAGGGa -3' miRNA: 3'- -UCUGUCGAAGGUCGUAGUGCUCUCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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