Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19686 | 5' | -58.9 | NC_004686.1 | + | 10747 | 0.66 | 0.526952 |
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Target: 5'- gGUugUUgCCG-GCACCCC-CGCGGc- -3' miRNA: 3'- -CAugAGgGGCaUGUGGGGuGCGCCua -5' |
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| 19686 | 5' | -58.9 | NC_004686.1 | + | 31256 | 0.66 | 0.526952 |
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Target: 5'- ----aCCCCGcGCACCCgAgGUGGGUg -3' miRNA: 3'- caugaGGGGCaUGUGGGgUgCGCCUA- -5' |
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| 19686 | 5' | -58.9 | NC_004686.1 | + | 27177 | 0.66 | 0.493143 |
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Target: 5'- -cACaUCCCUGUACggguaaucagcaccACCCCACGCcGAc -3' miRNA: 3'- caUG-AGGGGCAUG--------------UGGGGUGCGcCUa -5' |
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| 19686 | 5' | -58.9 | NC_004686.1 | + | 50556 | 0.67 | 0.45647 |
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Target: 5'- aUACUgaCCUGcACACCCCaacggaACGCGGAg -3' miRNA: 3'- cAUGAg-GGGCaUGUGGGG------UGCGCCUa -5' |
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| 19686 | 5' | -58.9 | NC_004686.1 | + | 32272 | 0.68 | 0.418535 |
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Target: 5'- -aACaCCCCGaUACACCUCGgGUGGGUc -3' miRNA: 3'- caUGaGGGGC-AUGUGGGGUgCGCCUA- -5' |
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| 19686 | 5' | -58.9 | NC_004686.1 | + | 43287 | 0.68 | 0.409354 |
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Target: 5'- cGUACagCUCCG-ACACCCCACccuCGGAc -3' miRNA: 3'- -CAUGa-GGGGCaUGUGGGGUGc--GCCUa -5' |
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| 19686 | 5' | -58.9 | NC_004686.1 | + | 6231 | 0.68 | 0.373916 |
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Target: 5'- -gACUCCCCGUACggcgccggucgGCCCgCAUG-GGAg -3' miRNA: 3'- caUGAGGGGCAUG-----------UGGG-GUGCgCCUa -5' |
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| 19686 | 5' | -58.9 | NC_004686.1 | + | 52065 | 0.68 | 0.370489 |
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Target: 5'- -gGCUCCaCCGgcaacgcuugacCACCCCACGcCGGGa -3' miRNA: 3'- caUGAGG-GGCau----------GUGGGGUGC-GCCUa -5' |
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| 19686 | 5' | -58.9 | NC_004686.1 | + | 31445 | 1.04 | 0.000935 |
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Target: 5'- gGUACUCCCCGUACACCCCACGCGGAUc -3' miRNA: 3'- -CAUGAGGGGCAUGUGGGGUGCGCCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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