Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19691 | 5' | -48.3 | NC_004686.1 | + | 25077 | 0.66 | 0.974165 |
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Target: 5'- gGAACCuCAGUGGucaacuGCCGc----GAGGUCg -3' miRNA: 3'- -CUUGG-GUCACC------UGGCuuuuuCUUCAG- -5' |
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| 19691 | 5' | -48.3 | NC_004686.1 | + | 41548 | 0.66 | 0.974165 |
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Target: 5'- --uCUCAGUGGAaUCGAu---GAAGUCg -3' miRNA: 3'- cuuGGGUCACCU-GGCUuuuuCUUCAG- -5' |
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| 19691 | 5' | -48.3 | NC_004686.1 | + | 25529 | 0.67 | 0.955924 |
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Target: 5'- uGACCCuGUGGAguuuCCGGAuccGAAGUUu -3' miRNA: 3'- cUUGGGuCACCU----GGCUUuuuCUUCAG- -5' |
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| 19691 | 5' | -48.3 | NC_004686.1 | + | 37133 | 0.67 | 0.95143 |
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Target: 5'- uGGCUguG-GaGGCUGAGGAAGAAGUCc -3' miRNA: 3'- cUUGGguCaC-CUGGCUUUUUCUUCAG- -5' |
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| 19691 | 5' | -48.3 | NC_004686.1 | + | 14927 | 0.69 | 0.911492 |
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Target: 5'- uGACCCAGUGGGCuCGGAuacac-GUCc -3' miRNA: 3'- cUUGGGUCACCUG-GCUUuuucuuCAG- -5' |
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| 19691 | 5' | -48.3 | NC_004686.1 | + | 34537 | 1.11 | 0.003611 |
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Target: 5'- uGAACCCAGUGGACCGAAAAAGAAGUCa -3' miRNA: 3'- -CUUGGGUCACCUGGCUUUUUCUUCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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