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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19692 | 3' | -52.8 | NC_004686.1 | + | 47806 | 0.66 | 0.843887 |
Target: 5'- gCGGCUaCGggcGCUUUGGCGcgGGGGcggaUGUCa -3' miRNA: 3'- -GUCGA-GC---UGAGACUGCuuUCCC----ACAG- -5' |
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19692 | 3' | -52.8 | NC_004686.1 | + | 7974 | 0.68 | 0.70226 |
Target: 5'- gGGCagCGGCUCcccgGACGAAucguguugcugcAGGGUGUg -3' miRNA: 3'- gUCGa-GCUGAGa---CUGCUU------------UCCCACAg -5' |
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19692 | 3' | -52.8 | NC_004686.1 | + | 34989 | 1.1 | 0.001384 |
Target: 5'- cCAGCUCGACUCUGACGAAAGGGUGUCg -3' miRNA: 3'- -GUCGAGCUGAGACUGCUUUCCCACAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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