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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19692 | 5' | -58.5 | NC_004686.1 | + | 24719 | 0.69 | 0.376671 |
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Target: 5'- gUCG-UACCCUcuuuuuaUGGGGGAGUCUgAGGAUc -3' miRNA: 3'- -AGCuGUGGGA-------ACCCCCUCGGG-UCUUG- -5' |
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| 19692 | 5' | -58.5 | NC_004686.1 | + | 41413 | 0.69 | 0.368935 |
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Target: 5'- -gGACACCCUUGgcuGGGGAguugacgugcuGCCCGGu-- -3' miRNA: 3'- agCUGUGGGAAC---CCCCU-----------CGGGUCuug -5' |
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| 19692 | 5' | -58.5 | NC_004686.1 | + | 10142 | 0.7 | 0.305053 |
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Target: 5'- cUUGACGCCgcCUUGGGGcGGCUCGGcGCg -3' miRNA: 3'- -AGCUGUGG--GAACCCCcUCGGGUCuUG- -5' |
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| 19692 | 5' | -58.5 | NC_004686.1 | + | 12448 | 0.7 | 0.305053 |
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Target: 5'- uUCGACGCCgaUGGcGcGGcGGCCCAGGuuGCg -3' miRNA: 3'- -AGCUGUGGgaACC-C-CC-UCGGGUCU--UG- -5' |
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| 19692 | 5' | -58.5 | NC_004686.1 | + | 29986 | 0.71 | 0.256414 |
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Target: 5'- -aGGCACC--UGGGGGAGUgggcaCCGGAACc -3' miRNA: 3'- agCUGUGGgaACCCCCUCG-----GGUCUUG- -5' |
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| 19692 | 5' | -58.5 | NC_004686.1 | + | 35023 | 1.1 | 0.000387 |
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Target: 5'- gUCGACACCCUUGGGGGAGCCCAGAACa -3' miRNA: 3'- -AGCUGUGGGAACCCCCUCGGGUCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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