Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19693 | 3' | -55 | NC_004686.1 | + | 31753 | 0.65 | 0.72247 |
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Target: 5'- -gGCGgc-CGCCGccGCaGCGCUucACGCa -3' miRNA: 3'- uaCGCauaGCGGCuuCG-CGCGA--UGUG- -5' |
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| 19693 | 3' | -55 | NC_004686.1 | + | 25443 | 0.65 | 0.72247 |
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Target: 5'- cAUGCGgcaagccuUGUCGCCGAccucCGCGUccgACACa -3' miRNA: 3'- -UACGC--------AUAGCGGCUuc--GCGCGa--UGUG- -5' |
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| 19693 | 3' | -55 | NC_004686.1 | + | 25444 | 0.65 | 0.72247 |
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Target: 5'- -aGUGguuUCGCCGAAcgauCGUGCUGCGg -3' miRNA: 3'- uaCGCau-AGCGGCUUc---GCGCGAUGUg -5' |
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| 19693 | 3' | -55 | NC_004686.1 | + | 45308 | 0.66 | 0.689661 |
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Target: 5'- -gGCGUGUacccCGCCGggGCaUGgaACACc -3' miRNA: 3'- uaCGCAUA----GCGGCuuCGcGCgaUGUG- -5' |
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| 19693 | 3' | -55 | NC_004686.1 | + | 21822 | 0.66 | 0.667483 |
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Target: 5'- uUGCGggugCGCCucGGCGgCGCggGCGCg -3' miRNA: 3'- uACGCaua-GCGGcuUCGC-GCGa-UGUG- -5' |
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| 19693 | 3' | -55 | NC_004686.1 | + | 29185 | 0.66 | 0.667483 |
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Target: 5'- uGUGgGUGUUGCUGuuguugguuGCGUGCgGCGCa -3' miRNA: 3'- -UACgCAUAGCGGCuu-------CGCGCGaUGUG- -5' |
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| 19693 | 3' | -55 | NC_004686.1 | + | 31205 | 0.67 | 0.645168 |
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Target: 5'- cGUGCGgccGUCGUCGcuGCGgGC-GCGCa -3' miRNA: 3'- -UACGCa--UAGCGGCuuCGCgCGaUGUG- -5' |
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| 19693 | 3' | -55 | NC_004686.1 | + | 34400 | 0.67 | 0.611629 |
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Target: 5'- aGUGCGUcgCGCCGAcagggguuGGgGCGUuggUGCGg -3' miRNA: 3'- -UACGCAuaGCGGCU--------UCgCGCG---AUGUg -5' |
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| 19693 | 3' | -55 | NC_004686.1 | + | 34068 | 0.7 | 0.440937 |
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Target: 5'- -aGCGgugcCGCCGAGGCcGCGC-GCGCc -3' miRNA: 3'- uaCGCaua-GCGGCUUCG-CGCGaUGUG- -5' |
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| 19693 | 3' | -55 | NC_004686.1 | + | 37416 | 1.08 | 0.000981 |
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Target: 5'- gAUGCGUAUCGCCGAAGCGCGCUACACg -3' miRNA: 3'- -UACGCAUAGCGGCUUCGCGCGAUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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