Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19694 | 3' | -51.6 | NC_004686.1 | + | 49210 | 0.7 | 0.764883 |
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Target: 5'- cCCGaGUUgAUCGCCGaaGUUGAGcgucUCCGg -3' miRNA: 3'- -GGC-CAAgUAGCGGCagUAGCUU----AGGC- -5' |
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| 19694 | 3' | -51.6 | NC_004686.1 | + | 33710 | 0.71 | 0.70154 |
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Target: 5'- aCCGGUgacggUCAUCGCCGcCGgggCGGggCUGa -3' miRNA: 3'- -GGCCA-----AGUAGCGGCaGUa--GCUuaGGC- -5' |
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| 19694 | 3' | -51.6 | NC_004686.1 | + | 6694 | 0.71 | 0.67976 |
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Target: 5'- -gGGUUCAguaCGaaGUCAUCGGAuaUCCGg -3' miRNA: 3'- ggCCAAGUa--GCggCAGUAGCUU--AGGC- -5' |
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| 19694 | 3' | -51.6 | NC_004686.1 | + | 50829 | 0.73 | 0.569785 |
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Target: 5'- gCUGGacUUCAaCGCCGacgaCGUCGAAUCCGu -3' miRNA: 3'- -GGCC--AAGUaGCGGCa---GUAGCUUAGGC- -5' |
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| 19694 | 3' | -51.6 | NC_004686.1 | + | 6326 | 0.74 | 0.526715 |
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Target: 5'- gCGGccUUCGUCGCCGgcgaGUCGggUuCCGa -3' miRNA: 3'- gGCC--AAGUAGCGGCag--UAGCuuA-GGC- -5' |
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| 19694 | 3' | -51.6 | NC_004686.1 | + | 48415 | 0.78 | 0.320601 |
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Target: 5'- gCGGUUCGagCGCCG-CAUCGAGUUCa -3' miRNA: 3'- gGCCAAGUa-GCGGCaGUAGCUUAGGc -5' |
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| 19694 | 3' | -51.6 | NC_004686.1 | + | 38477 | 1.12 | 0.001905 |
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Target: 5'- aCCGGUUCAUCGCCGUCAUCGAAUCCGa -3' miRNA: 3'- -GGCCAAGUAGCGGCAGUAGCUUAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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