Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19698 | 5' | -53.7 | NC_004686.1 | + | 467 | 0.67 | 0.749149 |
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Target: 5'- gAAUGAGgcgauggCCGCUGGUGAugagcuGGCGgagGCg -3' miRNA: 3'- -UUGCUCag-----GGUGAUCACUu-----CCGCa--CG- -5' |
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| 19698 | 5' | -53.7 | NC_004686.1 | + | 42751 | 0.67 | 0.749149 |
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Target: 5'- uGACG-GUCCUAC-GGUucaGGAGGCGUugGCg -3' miRNA: 3'- -UUGCuCAGGGUGaUCA---CUUCCGCA--CG- -5' |
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| 19698 | 5' | -53.7 | NC_004686.1 | + | 51495 | 0.67 | 0.72778 |
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Target: 5'- uAACGAuGUCUgAUgcucguGUGggGGCGUGg -3' miRNA: 3'- -UUGCU-CAGGgUGau----CACuuCCGCACg -5' |
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| 19698 | 5' | -53.7 | NC_004686.1 | + | 27125 | 0.67 | 0.72778 |
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Target: 5'- cGGCGAG-CCaGCUcgaacAG-GAAGGCGUGUg -3' miRNA: 3'- -UUGCUCaGGgUGA-----UCaCUUCCGCACG- -5' |
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| 19698 | 5' | -53.7 | NC_004686.1 | + | 2942 | 0.67 | 0.710395 |
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Target: 5'- cAGCGGGUgaaccauguacugcaCCUGCUGGUGucGGUGcUGCu -3' miRNA: 3'- -UUGCUCA---------------GGGUGAUCACuuCCGC-ACG- -5' |
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| 19698 | 5' | -53.7 | NC_004686.1 | + | 37802 | 0.7 | 0.560158 |
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Target: 5'- cGACGAG-CCCGCUGGcuuguucUGAgauAGGCGgGCc -3' miRNA: 3'- -UUGCUCaGGGUGAUC-------ACU---UCCGCaCG- -5' |
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| 19698 | 5' | -53.7 | NC_004686.1 | + | 19266 | 0.71 | 0.507093 |
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Target: 5'- cACGAGUUgCGgU-GUGAGGGCgGUGCg -3' miRNA: 3'- uUGCUCAGgGUgAuCACUUCCG-CACG- -5' |
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| 19698 | 5' | -53.7 | NC_004686.1 | + | 8582 | 0.75 | 0.303644 |
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Target: 5'- -uUGAGUCCCGCaAGUaccaGAAGGCGUugGCg -3' miRNA: 3'- uuGCUCAGGGUGaUCA----CUUCCGCA--CG- -5' |
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| 19698 | 5' | -53.7 | NC_004686.1 | + | 40160 | 1.1 | 0.001042 |
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Target: 5'- gAACGAGUCCCACUAGUGAAGGCGUGCa -3' miRNA: 3'- -UUGCUCAGGGUGAUCACUUCCGCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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