Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19699 | 3' | -47.4 | NC_004686.1 | + | 4258 | 0.66 | 0.988678 |
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Target: 5'- uGGGUgGAcuGGaUUGGGGGACGCGUgauGCGu -3' miRNA: 3'- -UUUAgCU--UC-AACUCCUUGCGCA---UGCu -5' |
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| 19699 | 3' | -47.4 | NC_004686.1 | + | 21385 | 0.66 | 0.987681 |
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Target: 5'- -cGUCGGAGUUGGGGGcggugucuggugcucGgCGuCGUGCGc -3' miRNA: 3'- uuUAGCUUCAACUCCU---------------U-GC-GCAUGCu -5' |
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| 19699 | 3' | -47.4 | NC_004686.1 | + | 19011 | 0.66 | 0.986979 |
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Target: 5'- ---cCGAAG-UGGGcGAACGCGUuuGAc -3' miRNA: 3'- uuuaGCUUCaACUC-CUUGCGCAugCU- -5' |
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| 19699 | 3' | -47.4 | NC_004686.1 | + | 23939 | 0.66 | 0.983001 |
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Target: 5'- -uAUCGggGUguucGAACGCGUucACGAu -3' miRNA: 3'- uuUAGCuuCAacucCUUGCGCA--UGCU- -5' |
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| 19699 | 3' | -47.4 | NC_004686.1 | + | 14556 | 0.67 | 0.980698 |
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Target: 5'- ----aGGAGUuguuUGAGGAACGCGgguCGu -3' miRNA: 3'- uuuagCUUCA----ACUCCUUGCGCau-GCu -5' |
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| 19699 | 3' | -47.4 | NC_004686.1 | + | 249 | 0.68 | 0.95767 |
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Target: 5'- uGGUCuGAGGaaGAGGcuGACGCGUugGAc -3' miRNA: 3'- uUUAG-CUUCaaCUCC--UUGCGCAugCU- -5' |
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| 19699 | 3' | -47.4 | NC_004686.1 | + | 960 | 0.72 | 0.842014 |
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Target: 5'- ---aUGAGGUUGAGGGAUugGCGggagACGAu -3' miRNA: 3'- uuuaGCUUCAACUCCUUG--CGCa---UGCU- -5' |
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| 19699 | 3' | -47.4 | NC_004686.1 | + | 41617 | 1.08 | 0.00841 |
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Target: 5'- gAAAUCGAAGUUGAGGAACGCGUACGAc -3' miRNA: 3'- -UUUAGCUUCAACUCCUUGCGCAUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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