Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19704 | 5' | -56.9 | NC_004686.1 | + | 6153 | 0.66 | 0.681999 |
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Target: 5'- ----cGGcGCGGUgaUGCGCUGGUCGCaagCCg -3' miRNA: 3'- ccagaUC-CGCCA--GCGUGACCAGUG---GG- -5' |
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| 19704 | 5' | -56.9 | NC_004686.1 | + | 1583 | 0.67 | 0.606835 |
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Target: 5'- cGGUCaUGGuGCuGUCGUACaGGUCGCa- -3' miRNA: 3'- -CCAG-AUC-CGcCAGCGUGaCCAGUGgg -5' |
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| 19704 | 5' | -56.9 | NC_004686.1 | + | 192 | 0.67 | 0.574751 |
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Target: 5'- cGGg-UGGGCGacggugccguuGUCGCugUGGUCgACCa -3' miRNA: 3'- -CCagAUCCGC-----------CAGCGugACCAG-UGGg -5' |
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| 19704 | 5' | -56.9 | NC_004686.1 | + | 5157 | 0.67 | 0.574751 |
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Target: 5'- cGGccUCUAGGCuGUCGUGCUccuGGauccacUCGCCCc -3' miRNA: 3'- -CC--AGAUCCGcCAGCGUGA---CC------AGUGGG- -5' |
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| 19704 | 5' | -56.9 | NC_004686.1 | + | 15582 | 0.68 | 0.543071 |
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Target: 5'- uGGUCgaacguGGCGGcgUCGCAaccGGUguCCCa -3' miRNA: 3'- -CCAGau----CCGCC--AGCGUga-CCAguGGG- -5' |
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| 19704 | 5' | -56.9 | NC_004686.1 | + | 8899 | 0.68 | 0.532635 |
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Target: 5'- gGGUCgUAGGCGG-CGCACU--UCGCa- -3' miRNA: 3'- -CCAG-AUCCGCCaGCGUGAccAGUGgg -5' |
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| 19704 | 5' | -56.9 | NC_004686.1 | + | 8071 | 0.68 | 0.511987 |
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Target: 5'- uGGUCgacgGGGUGaagaccCGCauGCUGGUCAUCCc -3' miRNA: 3'- -CCAGa---UCCGCca----GCG--UGACCAGUGGG- -5' |
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| 19704 | 5' | -56.9 | NC_004686.1 | + | 33340 | 0.69 | 0.459015 |
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Target: 5'- cGGUCgAGGCGGUgauggugaaguuggCGaCACUGGagGCUCc -3' miRNA: 3'- -CCAGaUCCGCCA--------------GC-GUGACCagUGGG- -5' |
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| 19704 | 5' | -56.9 | NC_004686.1 | + | 43615 | 1.13 | 0.000413 |
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Target: 5'- gGGUCUAGGCGGUCGCACUGGUCACCCu -3' miRNA: 3'- -CCAGAUCCGCCAGCGUGACCAGUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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