Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19706 | 3' | -48.3 | NC_004686.1 | + | 3247 | 0.66 | 0.962224 |
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Target: 5'- aGGCgg--GUUUCGUCUuugGGugGGu- -3' miRNA: 3'- aCCGaaaaCAAAGCAGGa--CCugCUuu -5' |
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| 19706 | 3' | -48.3 | NC_004686.1 | + | 34340 | 0.68 | 0.921102 |
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Target: 5'- cUGGCUcgacUGaugaUCGUCCaGGACGAAAc -3' miRNA: 3'- -ACCGAaa--ACaa--AGCAGGaCCUGCUUU- -5' |
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| 19706 | 3' | -48.3 | NC_004686.1 | + | 21664 | 0.69 | 0.877308 |
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Target: 5'- aUGGUg---Gg--CGUCCUGGugGGGAa -3' miRNA: 3'- -ACCGaaaaCaaaGCAGGACCugCUUU- -5' |
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| 19706 | 3' | -48.3 | NC_004686.1 | + | 21843 | 0.81 | 0.27442 |
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Target: 5'- aGcGCUgc-GUUUCGUCCUGGACGAu- -3' miRNA: 3'- aC-CGAaaaCAAAGCAGGACCUGCUuu -5' |
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| 19706 | 3' | -48.3 | NC_004686.1 | + | 12199 | 0.88 | 0.11271 |
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Target: 5'- -cGCUUUUGUUUCGUCCaGGACGAAAc -3' miRNA: 3'- acCGAAAACAAAGCAGGaCCUGCUUU- -5' |
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| 19706 | 3' | -48.3 | NC_004686.1 | + | 43988 | 1.07 | 0.006375 |
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Target: 5'- aUGGCUUUUGUUUCGUCCUGGACGAAAc -3' miRNA: 3'- -ACCGAAAACAAAGCAGGACCUGCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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