Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19714 | 3' | -52.5 | NC_004686.1 | + | 41680 | 0.66 | 0.871903 |
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Target: 5'- aGCgGAGGUugGCGCcgcgccacGGcUCCGGAUc -3' miRNA: 3'- cCGaCUUCAugCGCGaa------CC-AGGCUUG- -5' |
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| 19714 | 3' | -52.5 | NC_004686.1 | + | 43719 | 0.66 | 0.871903 |
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Target: 5'- uGCgGggGUAgGCGCccccGGUCCcGGCg -3' miRNA: 3'- cCGaCuuCAUgCGCGaa--CCAGGcUUG- -5' |
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| 19714 | 3' | -52.5 | NC_004686.1 | + | 17764 | 0.66 | 0.855357 |
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Target: 5'- gGGCgUGggGUGCG-GCga-GUCCGGGg -3' miRNA: 3'- -CCG-ACuuCAUGCgCGaacCAGGCUUg -5' |
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| 19714 | 3' | -52.5 | NC_004686.1 | + | 50875 | 0.66 | 0.846717 |
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Target: 5'- uGGCUGAGGUGC-CGg-UGGUCagcuGACg -3' miRNA: 3'- -CCGACUUCAUGcGCgaACCAGgc--UUG- -5' |
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| 19714 | 3' | -52.5 | NC_004686.1 | + | 301 | 0.66 | 0.837842 |
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Target: 5'- aGGCUGuugagcaugucGGGUAUGUGgUUGGUgUGGAUg -3' miRNA: 3'- -CCGAC-----------UUCAUGCGCgAACCAgGCUUG- -5' |
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| 19714 | 3' | -52.5 | NC_004686.1 | + | 41422 | 0.66 | 0.837842 |
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Target: 5'- uGGCUGggGaguugACGUGCUgcccGGUCUccACc -3' miRNA: 3'- -CCGACuuCa----UGCGCGAa---CCAGGcuUG- -5' |
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| 19714 | 3' | -52.5 | NC_004686.1 | + | 18599 | 0.67 | 0.819431 |
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Target: 5'- aGGCUacGGGUGCGCGUgugcgGGUgcaucacucaCCGAACc -3' miRNA: 3'- -CCGAc-UUCAUGCGCGaa---CCA----------GGCUUG- -5' |
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| 19714 | 3' | -52.5 | NC_004686.1 | + | 49280 | 1.12 | 0.001289 |
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Target: 5'- cGGCUGAAGUACGCGCUUGGUCCGAACc -3' miRNA: 3'- -CCGACUUCAUGCGCGAACCAGGCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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