Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19716 | 5' | -59.3 | NC_004686.1 | + | 19207 | 0.7 | 0.268148 |
Target: 5'- -cGGGuGUGGGCGGcAAUGGUg-GCGAc -3' miRNA: 3'- guCCC-CACCCGCC-UUACCGagUGCUu -5' |
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19716 | 5' | -59.3 | NC_004686.1 | + | 9322 | 0.7 | 0.2595 |
Target: 5'- gGGGGGUgagucguacgguccGGGCGG--UGGCUCggGCGGAa -3' miRNA: 3'- gUCCCCA--------------CCCGCCuuACCGAG--UGCUU- -5' |
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19716 | 5' | -59.3 | NC_004686.1 | + | 36196 | 0.71 | 0.224251 |
Target: 5'- gCAGGcGGUGGGCGGGccugucauuUGGCUUgGCGAc -3' miRNA: 3'- -GUCC-CCACCCGCCUu--------ACCGAG-UGCUu -5' |
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19716 | 5' | -59.3 | NC_004686.1 | + | 23006 | 0.72 | 0.212881 |
Target: 5'- -uGGGGUGuGGCcGggUGGUUCACGc- -3' miRNA: 3'- guCCCCAC-CCGcCuuACCGAGUGCuu -5' |
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19716 | 5' | -59.3 | NC_004686.1 | + | 50185 | 1.05 | 0.000655 |
Target: 5'- uCAGGGGUGGGCGGAAUGGCUCACGAAa -3' miRNA: 3'- -GUCCCCACCCGCCUUACCGAGUGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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