Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19720 | 5' | -58.4 | NC_004686.1 | + | 18283 | 0.66 | 0.534491 |
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Target: 5'- cAGCACGGCAGC---GGGGCgaGC-CUCc -3' miRNA: 3'- -UCGUGCUGUCGgauCUCCGg-CGuGAG- -5' |
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| 19720 | 5' | -58.4 | NC_004686.1 | + | 34058 | 0.67 | 0.452809 |
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Target: 5'- cGGUgAUGACAgcgguGCCgccGAGGCCGCGCg- -3' miRNA: 3'- -UCG-UGCUGU-----CGGau-CUCCGGCGUGag -5' |
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| 19720 | 5' | -58.4 | NC_004686.1 | + | 12459 | 0.67 | 0.443071 |
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Target: 5'- uGGCGCGGCGGCCcAGGuugcGuGCCGCGa-- -3' miRNA: 3'- -UCGUGCUGUCGGaUCU----C-CGGCGUgag -5' |
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| 19720 | 5' | -58.4 | NC_004686.1 | + | 26434 | 0.68 | 0.414585 |
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Target: 5'- uGCGCGACA-CCUGc--GCUGCGCUCg -3' miRNA: 3'- uCGUGCUGUcGGAUcucCGGCGUGAG- -5' |
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| 19720 | 5' | -58.4 | NC_004686.1 | + | 49163 | 0.68 | 0.414585 |
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Target: 5'- uGCGCGACGgggacGCCgAGuucaucgcccAGGCgCGCACUCu -3' miRNA: 3'- uCGUGCUGU-----CGGaUC----------UCCG-GCGUGAG- -5' |
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| 19720 | 5' | -58.4 | NC_004686.1 | + | 22126 | 0.69 | 0.336266 |
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Target: 5'- uGGCGCGcGCGGCCUcGGcGGCacCGCugUCa -3' miRNA: 3'- -UCGUGC-UGUCGGA-UCuCCG--GCGugAG- -5' |
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| 19720 | 5' | -58.4 | NC_004686.1 | + | 6609 | 0.71 | 0.262566 |
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Target: 5'- gGGCACGACAGggUAGAGG-UGCACaUCg -3' miRNA: 3'- -UCGUGCUGUCggAUCUCCgGCGUG-AG- -5' |
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| 19720 | 5' | -58.4 | NC_004686.1 | + | 8046 | 0.72 | 0.236367 |
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Target: 5'- cGGCACGACAccGCCUccAGGacacgcggaucauCCGCGCUCg -3' miRNA: 3'- -UCGUGCUGU--CGGAucUCC-------------GGCGUGAG- -5' |
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| 19720 | 5' | -58.4 | NC_004686.1 | + | 51043 | 1.1 | 0.00036 |
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Target: 5'- gAGCACGACAGCCUAGAGGCCGCACUCa -3' miRNA: 3'- -UCGUGCUGUCGGAUCUCCGGCGUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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