miRNA: 3'- -GGGGCUGCUCAuagCC-CGCAaGACGG- -5'
miRNA: 3'- -GGGGCugCUCA---UAGCCCGcaAGAcGG- -5'
miRNA: 3'- -GGGGCUGCUCAu--AGC-CCGcaaGACGG- -5'
miRNA: 3'- -GGGGCUGCUCAUAGCCCGCAAGACGG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19721 | 3' | -55.7 | NC_004686.1 | + | 11956 | 0.73 | 0.355174 |
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Target: 5'- gCCCCGGCGAGU---GGaGCGU-UUGCCc -3' miRNA: 3'- -GGGGCUGCUCAuagCC-CGCAaGACGG- -5' |
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| 19721 | 3' | -55.7 | NC_004686.1 | + | 27322 | 0.73 | 0.355174 |
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Target: 5'- aCCCCGugGGGUgggAUCGGGU--UCUcCCa -3' miRNA: 3'- -GGGGCugCUCA---UAGCCCGcaAGAcGG- -5' |
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| 19721 | 3' | -55.7 | NC_004686.1 | + | 10728 | 0.73 | 0.346969 |
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Target: 5'- uCCCCGACGGGUcauUCGaGGUug-UUGCCg -3' miRNA: 3'- -GGGGCUGCUCAu--AGC-CCGcaaGACGG- -5' |
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| 19721 | 3' | -55.7 | NC_004686.1 | + | 51588 | 1.15 | 0.000483 |
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Target: 5'- aCCCCGACGAGUAUCGGGCGUUCUGCCa -3' miRNA: 3'- -GGGGCUGCUCAUAGCCCGCAAGACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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