Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19722 | 5' | -47.6 | NC_004686.1 | + | 17368 | 0.71 | 0.881972 |
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Target: 5'- -aGAUcuUGCGGGCggcGUCGuugacGUAGugGCCg -3' miRNA: 3'- ugCUA--ACGUCCGa--UAGC-----UAUUugCGG- -5' |
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| 19722 | 5' | -47.6 | NC_004686.1 | + | 290 | 0.74 | 0.713184 |
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Target: 5'- uGCGGggcgGCAGGCUGUUGAgc-AUGUCg -3' miRNA: 3'- -UGCUaa--CGUCCGAUAGCUauuUGCGG- -5' |
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| 19722 | 5' | -47.6 | NC_004686.1 | + | 1230 | 0.75 | 0.678895 |
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Target: 5'- cGCGGUUGUAGGCggcgcugcgGUUGAacUGGGCGUCu -3' miRNA: 3'- -UGCUAACGUCCGa--------UAGCU--AUUUGCGG- -5' |
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| 19722 | 5' | -47.6 | NC_004686.1 | + | 4157 | 0.78 | 0.485186 |
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Target: 5'- -gGGUUGCGGGUUGUCGAccgauGCGUCg -3' miRNA: 3'- ugCUAACGUCCGAUAGCUauu--UGCGG- -5' |
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| 19722 | 5' | -47.6 | NC_004686.1 | + | 49141 | 0.79 | 0.463827 |
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Target: 5'- -gGAUUGCAcguGGCUGUUGAUcugcgcgacggGGACGCCg -3' miRNA: 3'- ugCUAACGU---CCGAUAGCUA-----------UUUGCGG- -5' |
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| 19722 | 5' | -47.6 | NC_004686.1 | + | 52075 | 1.14 | 0.002988 |
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Target: 5'- aACGAUUGCAGGCUAUCGAUAAACGCCa -3' miRNA: 3'- -UGCUAACGUCCGAUAGCUAUUUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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