Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19731 | 3' | -54.7 | NC_004686.1 | + | 36061 | 0.66 | 0.712095 |
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Target: 5'- --aUGGCGaaucuugaucUUGUCGGCCugGUCgauaUCACu -3' miRNA: 3'- cuaGCCGU----------AGCAGCCGGugUAG----AGUG- -5' |
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| 19731 | 3' | -54.7 | NC_004686.1 | + | 41956 | 0.67 | 0.690342 |
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Target: 5'- -uUCGGaCAggGUCGGCCGCAcCUUg- -3' miRNA: 3'- cuAGCC-GUagCAGCCGGUGUaGAGug -5' |
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| 19731 | 3' | -54.7 | NC_004686.1 | + | 55540 | 0.69 | 0.568933 |
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Target: 5'- cGAUCGGCAUCGaaaGCCACAacCUUGCc -3' miRNA: 3'- -CUAGCCGUAGCagcCGGUGUa-GAGUG- -5' |
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| 19731 | 3' | -54.7 | NC_004686.1 | + | 28926 | 0.69 | 0.558027 |
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Target: 5'- cGUUGGCGgucgaaUCGaUUGGCCGCGUCuuuuUCACg -3' miRNA: 3'- cUAGCCGU------AGC-AGCCGGUGUAG----AGUG- -5' |
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| 19731 | 3' | -54.7 | NC_004686.1 | + | 5237 | 0.69 | 0.536404 |
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Target: 5'- uGAUCGGCAUCcaCGGCaucaACccgCUCACc -3' miRNA: 3'- -CUAGCCGUAGcaGCCGg---UGua-GAGUG- -5' |
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| 19731 | 3' | -54.7 | NC_004686.1 | + | 36659 | 0.69 | 0.536404 |
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Target: 5'- --cCGGCggCGUCGGCgGCGUCguaUCAa -3' miRNA: 3'- cuaGCCGuaGCAGCCGgUGUAG---AGUg -5' |
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| 19731 | 3' | -54.7 | NC_004686.1 | + | 50413 | 0.7 | 0.473535 |
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Target: 5'- cGUCGGCGUCG-CGGaCCACAgCgaaCGCa -3' miRNA: 3'- cUAGCCGUAGCaGCC-GGUGUaGa--GUG- -5' |
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| 19731 | 3' | -54.7 | NC_004686.1 | + | 28268 | 0.76 | 0.216949 |
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Target: 5'- -cUCGGCGUUGUCGGCguCGUgCUCAUc -3' miRNA: 3'- cuAGCCGUAGCAGCCGguGUA-GAGUG- -5' |
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| 19731 | 3' | -54.7 | NC_004686.1 | + | 29122 | 0.78 | 0.170104 |
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Target: 5'- gGAUCGGCGUCGgcgGGCCGCG-CUUGCg -3' miRNA: 3'- -CUAGCCGUAGCag-CCGGUGUaGAGUG- -5' |
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| 19731 | 3' | -54.7 | NC_004686.1 | + | 55519 | 1.1 | 0.000872 |
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Target: 5'- cGAUCGGCAUCGUCGGCCACAUCUCACg -3' miRNA: 3'- -CUAGCCGUAGCAGCCGGUGUAGAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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