Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19733 | 3' | -56.6 | NC_004686.1 | + | 50590 | 0.66 | 0.635737 |
Target: 5'- uGCCGauCACa-UUGACCGGGCCAAAg -3' miRNA: 3'- gCGGCacGUGgaGACUGGUCUGGUUU- -5' |
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19733 | 3' | -56.6 | NC_004686.1 | + | 5890 | 0.66 | 0.590558 |
Target: 5'- uCGCCGaguccaagaaggaUGCGCgUCUGGCCGGugGCUAu- -3' miRNA: 3'- -GCGGC-------------ACGUGgAGACUGGUC--UGGUuu -5' |
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19733 | 3' | -56.6 | NC_004686.1 | + | 34972 | 0.67 | 0.54704 |
Target: 5'- aCGCCGUggcccagGCACCgcugCUGACCGacACCAc- -3' miRNA: 3'- -GCGGCA-------CGUGGa---GACUGGUc-UGGUuu -5' |
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19733 | 3' | -56.6 | NC_004686.1 | + | 27109 | 0.68 | 0.505635 |
Target: 5'- gGuCCGUGCGCCUCgacGGCgAG-CCAGc -3' miRNA: 3'- gC-GGCACGUGGAGa--CUGgUCuGGUUu -5' |
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19733 | 3' | -56.6 | NC_004686.1 | + | 49098 | 0.72 | 0.290235 |
Target: 5'- cCGCCGUGCACC-CUG-CCGGcUCGAc -3' miRNA: 3'- -GCGGCACGUGGaGACuGGUCuGGUUu -5' |
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19733 | 3' | -56.6 | NC_004686.1 | + | 56094 | 1.06 | 0.001015 |
Target: 5'- aCGCCGUGCACCUCUGACCAGACCAAAc -3' miRNA: 3'- -GCGGCACGUGGAGACUGGUCUGGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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