Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19739 | 3' | -53.9 | NC_004687.1 | + | 88129 | 0.69 | 0.830953 |
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Target: 5'- gGGUGGCCgaGcaugucggcgacGUUGAcccgGUGGUUGCCg -3' miRNA: 3'- aUCGCCGGgaC------------CAACUa---CACUAACGG- -5' |
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| 19739 | 3' | -53.9 | NC_004687.1 | + | 80680 | 0.69 | 0.822281 |
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Target: 5'- gAGCGGCCauuCUGGUUGAUcucg--GCCa -3' miRNA: 3'- aUCGCCGG---GACCAACUAcacuaaCGG- -5' |
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| 19739 | 3' | -53.9 | NC_004687.1 | + | 12896 | 0.71 | 0.737134 |
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Target: 5'- -cGCaGGCCCgucgGGUUGAagGUGAagguggUGCCa -3' miRNA: 3'- auCG-CCGGGa---CCAACUa-CACUa-----ACGG- -5' |
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| 19739 | 3' | -53.9 | NC_004687.1 | + | 91132 | 0.72 | 0.696362 |
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Target: 5'- -cGgGGCCCcGGcgacgaccaaGAUGUGGUUGCCg -3' miRNA: 3'- auCgCCGGGaCCaa--------CUACACUAACGG- -5' |
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| 19739 | 3' | -53.9 | NC_004687.1 | + | 45531 | 0.72 | 0.696362 |
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Target: 5'- cAGCGGCCa-GGUUGGUgGUGAUcaagGUCg -3' miRNA: 3'- aUCGCCGGgaCCAACUA-CACUAa---CGG- -5' |
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| 19739 | 3' | -53.9 | NC_004687.1 | + | 136849 | 0.72 | 0.686003 |
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Target: 5'- cGGCGGCaCCUGGUcGGUGg---UGCUg -3' miRNA: 3'- aUCGCCG-GGACCAaCUACacuaACGG- -5' |
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| 19739 | 3' | -53.9 | NC_004687.1 | + | 155330 | 0.72 | 0.686003 |
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Target: 5'- cUGGUGGCCCUGGUgc---UGAgccUGCCg -3' miRNA: 3'- -AUCGCCGGGACCAacuacACUa--ACGG- -5' |
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| 19739 | 3' | -53.9 | NC_004687.1 | + | 133325 | 0.72 | 0.675598 |
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Target: 5'- -uGCGGCCCUccuuucggugGGUggUGGUaGUGggUGCCg -3' miRNA: 3'- auCGCCGGGA----------CCA--ACUA-CACuaACGG- -5' |
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| 19739 | 3' | -53.9 | NC_004687.1 | + | 148194 | 1.11 | 0.002721 |
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Target: 5'- aUAGCGGCCCUGGUUGAUGUGAUUGCCg -3' miRNA: 3'- -AUCGCCGGGACCAACUACACUAACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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