Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19745 | 3' | -51.6 | NC_004687.1 | + | 32129 | 0.7 | 0.917266 |
Target: 5'- cAGAUC-UUCgCCGAGGUgccgGUGGUCg -3' miRNA: 3'- -UCUAGuAAGgGGCUCCGua--CACUAGg -5' |
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19745 | 3' | -51.6 | NC_004687.1 | + | 131337 | 0.7 | 0.904985 |
Target: 5'- gAGAUCGUcggauUCaCCUcGGGCggGUGGUCCu -3' miRNA: 3'- -UCUAGUA-----AG-GGGcUCCGuaCACUAGG- -5' |
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19745 | 3' | -51.6 | NC_004687.1 | + | 58016 | 0.71 | 0.891716 |
Target: 5'- cGGGUCAgcucguagUCCCCGgcgGGGCcaGUGAUCUu -3' miRNA: 3'- -UCUAGUa-------AGGGGC---UCCGuaCACUAGG- -5' |
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19745 | 3' | -51.6 | NC_004687.1 | + | 142610 | 0.72 | 0.854427 |
Target: 5'- cGGGUCAgugagCCCGcAGG-AUGUGAUCCa -3' miRNA: 3'- -UCUAGUaag--GGGC-UCCgUACACUAGG- -5' |
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19745 | 3' | -51.6 | NC_004687.1 | + | 84389 | 0.75 | 0.694462 |
Target: 5'- cGGU-GUUCCUgGAGGC-UGUGAUCCu -3' miRNA: 3'- uCUAgUAAGGGgCUCCGuACACUAGG- -5' |
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19745 | 3' | -51.6 | NC_004687.1 | + | 64520 | 0.8 | 0.422024 |
Target: 5'- cGGGUCAUUCCCCGGuGuuAUGUGAUCg -3' miRNA: 3'- -UCUAGUAAGGGGCU-CcgUACACUAGg -5' |
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19745 | 3' | -51.6 | NC_004687.1 | + | 140721 | 1.14 | 0.004017 |
Target: 5'- gAGAUCAUUCCCCGAGGCAUGUGAUCCg -3' miRNA: 3'- -UCUAGUAAGGGGCUCCGUACACUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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