Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19752 | 5' | -51.3 | NC_004687.1 | + | 122112 | 0.7 | 0.907178 |
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Target: 5'- --aCCgGGGCGucGCGGUCCUCagCGa -3' miRNA: 3'- aaaGGgUCCGCuuUGCUAGGAGaaGU- -5' |
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| 19752 | 5' | -51.3 | NC_004687.1 | + | 64140 | 0.71 | 0.871251 |
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Target: 5'- -gUCCCGGGCGccGuugaggcgcucccAGCGGUCCUCcUCGg -3' miRNA: 3'- aaAGGGUCCGC--U-------------UUGCUAGGAGaAGU- -5' |
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| 19752 | 5' | -51.3 | NC_004687.1 | + | 73937 | 0.73 | 0.803086 |
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Target: 5'- ---gCCAGGCGGugaacccGGCGAUCCUCggCAu -3' miRNA: 3'- aaagGGUCCGCU-------UUGCUAGGAGaaGU- -5' |
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| 19752 | 5' | -51.3 | NC_004687.1 | + | 54258 | 0.73 | 0.765667 |
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Target: 5'- -gUCCCAGGaCaAGGCGAUCgUCUUCu -3' miRNA: 3'- aaAGGGUCC-GcUUUGCUAGgAGAAGu -5' |
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| 19752 | 5' | -51.3 | NC_004687.1 | + | 135906 | 0.77 | 0.576997 |
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Target: 5'- -cUCCCAGGUGAucuCGcuggaGUCCUCUUCGu -3' miRNA: 3'- aaAGGGUCCGCUuu-GC-----UAGGAGAAGU- -5' |
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| 19752 | 5' | -51.3 | NC_004687.1 | + | 80756 | 0.77 | 0.576997 |
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Target: 5'- --cCCCGGGCGAGGCGGgcacCCUCUaCAu -3' miRNA: 3'- aaaGGGUCCGCUUUGCUa---GGAGAaGU- -5' |
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| 19752 | 5' | -51.3 | NC_004687.1 | + | 132834 | 1.06 | 0.010984 |
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Target: 5'- cUUUCCCAGGCGAAACGAUCCUCUUCAc -3' miRNA: 3'- -AAAGGGUCCGCUUUGCUAGGAGAAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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