Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19825 | 3' | -51 | NC_004687.1 | + | 37247 | 0.66 | 0.992981 |
Target: 5'- aCCGUGcucagcgagGCGCCGccgccGcCGAGCUUGaUCa -3' miRNA: 3'- -GGUACaa-------CGCGGU-----CaGCUCGAACaAG- -5' |
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19825 | 3' | -51 | NC_004687.1 | + | 113973 | 0.66 | 0.992981 |
Target: 5'- aCCAUGc--CGCCGGUCugcuuGGCcUGUUCg -3' miRNA: 3'- -GGUACaacGCGGUCAGc----UCGaACAAG- -5' |
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19825 | 3' | -51 | NC_004687.1 | + | 106908 | 0.67 | 0.987951 |
Target: 5'- gCAUGggugGCGCCGGggCGGGCgcggGUg- -3' miRNA: 3'- gGUACaa--CGCGGUCa-GCUCGaa--CAag -5' |
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19825 | 3' | -51 | NC_004687.1 | + | 99732 | 0.67 | 0.982621 |
Target: 5'- gCAUGUg--GCCGGgcUCGGGCaUGUUCg -3' miRNA: 3'- gGUACAacgCGGUC--AGCUCGaACAAG- -5' |
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19825 | 3' | -51 | NC_004687.1 | + | 93229 | 0.67 | 0.980498 |
Target: 5'- ---cGUUGCGCUGGUacgcagccgaCGAGCU-GUUCc -3' miRNA: 3'- gguaCAACGCGGUCA----------GCUCGAaCAAG- -5' |
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19825 | 3' | -51 | NC_004687.1 | + | 29864 | 0.68 | 0.978189 |
Target: 5'- gCAUGUUGgGCCGGUgGAuGCUg---- -3' miRNA: 3'- gGUACAACgCGGUCAgCU-CGAacaag -5' |
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19825 | 3' | -51 | NC_004687.1 | + | 8025 | 0.68 | 0.972972 |
Target: 5'- ---cGggGUGCCcuuggGGUCGGGCUUGUa- -3' miRNA: 3'- gguaCaaCGCGG-----UCAGCUCGAACAag -5' |
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19825 | 3' | -51 | NC_004687.1 | + | 66893 | 0.7 | 0.943121 |
Target: 5'- aCCGUGgagGUGUCGGUC-AGCUUGgaCa -3' miRNA: 3'- -GGUACaa-CGCGGUCAGcUCGAACaaG- -5' |
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19825 | 3' | -51 | NC_004687.1 | + | 100047 | 0.7 | 0.927863 |
Target: 5'- gCCGUa--GCGCCAGUCcGGCUugccggUGUUCg -3' miRNA: 3'- -GGUAcaaCGCGGUCAGcUCGA------ACAAG- -5' |
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19825 | 3' | -51 | NC_004687.1 | + | 61678 | 0.72 | 0.875993 |
Target: 5'- cCCGUGUUGC-CCuguAGUCGAGCUa---- -3' miRNA: 3'- -GGUACAACGcGG---UCAGCUCGAacaag -5' |
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19825 | 3' | -51 | NC_004687.1 | + | 149487 | 0.72 | 0.844321 |
Target: 5'- gCAUGaccGCGCC-GUCGGGCUcguagUGUUCg -3' miRNA: 3'- gGUACaa-CGCGGuCAGCUCGA-----ACAAG- -5' |
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19825 | 3' | -51 | NC_004687.1 | + | 15136 | 0.74 | 0.781268 |
Target: 5'- -gGUGUUGaCGCCGGUCGGGCg----- -3' miRNA: 3'- ggUACAAC-GCGGUCAGCUCGaacaag -5' |
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19825 | 3' | -51 | NC_004687.1 | + | 91374 | 0.74 | 0.771609 |
Target: 5'- gCAUGaugGCGCCGGUCGAGCcc-UUCc -3' miRNA: 3'- gGUACaa-CGCGGUCAGCUCGaacAAG- -5' |
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19825 | 3' | -51 | NC_004687.1 | + | 7022 | 0.76 | 0.679885 |
Target: 5'- cCCAUGUuccgcUGCGcCCGGcUGAGCUUGUUg -3' miRNA: 3'- -GGUACA-----ACGC-GGUCaGCUCGAACAAg -5' |
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19825 | 3' | -51 | NC_004687.1 | + | 42356 | 0.78 | 0.584655 |
Target: 5'- gCgAUGUUGCGCaccCGGUUGAGCUUGUcCa -3' miRNA: 3'- -GgUACAACGCG---GUCAGCUCGAACAaG- -5' |
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19825 | 3' | -51 | NC_004687.1 | + | 77547 | 1.13 | 0.004875 |
Target: 5'- uCCAUGUUGCGCCAGUCGAGCUUGUUCa -3' miRNA: 3'- -GGUACAACGCGGUCAGCUCGAACAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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