Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19848 | 3' | -51.1 | NC_004687.1 | + | 118902 | 0.68 | 0.964087 |
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Target: 5'- gCGCCGucGGcGAGAgCCA-GGAGGAGCg -3' miRNA: 3'- -GUGGUcuCC-CUCUaGGUaCUUCUUCG- -5' |
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| 19848 | 3' | -51.1 | NC_004687.1 | + | 17600 | 0.69 | 0.948409 |
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Target: 5'- gACCGGcAGGGGGuucaucgguAUCCG-GGAGAAGUu -3' miRNA: 3'- gUGGUC-UCCCUC---------UAGGUaCUUCUUCG- -5' |
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| 19848 | 3' | -51.1 | NC_004687.1 | + | 60830 | 0.69 | 0.943409 |
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Target: 5'- uGCCGGAGGGAGGUCuCGaaccuccUGAccGAccGGCg -3' miRNA: 3'- gUGGUCUCCCUCUAG-GU-------ACUu-CU--UCG- -5' |
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| 19848 | 3' | -51.1 | NC_004687.1 | + | 155704 | 0.7 | 0.93909 |
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Target: 5'- uGCCGacGAGGu-GAUCUuUGAGGAAGCg -3' miRNA: 3'- gUGGU--CUCCcuCUAGGuACUUCUUCG- -5' |
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| 19848 | 3' | -51.1 | NC_004687.1 | + | 85591 | 0.71 | 0.89162 |
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Target: 5'- aCGCCGGAGccGGGUCguUGAAGAAGg -3' miRNA: 3'- -GUGGUCUCccUCUAGguACUUCUUCg -5' |
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| 19848 | 3' | -51.1 | NC_004687.1 | + | 103815 | 0.73 | 0.801776 |
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Target: 5'- gCACCgugugcuugugGGAGGGGGGUuuGgggGGAGggGCa -3' miRNA: 3'- -GUGG-----------UCUCCCUCUAggUa--CUUCuuCG- -5' |
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| 19848 | 3' | -51.1 | NC_004687.1 | + | 46198 | 0.74 | 0.763577 |
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Target: 5'- gGCCGagcugauucgcGAGGGAGA-CCGUGgcGggGCc -3' miRNA: 3'- gUGGU-----------CUCCCUCUaGGUACuuCuuCG- -5' |
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| 19848 | 3' | -51.1 | NC_004687.1 | + | 62278 | 0.74 | 0.743686 |
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Target: 5'- cCGCgUAGAGGGAG-UCCAUGuccGGAGCc -3' miRNA: 3'- -GUG-GUCUCCCUCuAGGUACuu-CUUCG- -5' |
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| 19848 | 3' | -51.1 | NC_004687.1 | + | 111899 | 0.75 | 0.71309 |
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Target: 5'- gCAUCAGAucGuGGAGGUCCAUGAGGucGUc -3' miRNA: 3'- -GUGGUCU--C-CCUCUAGGUACUUCuuCG- -5' |
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| 19848 | 3' | -51.1 | NC_004687.1 | + | 64981 | 1.13 | 0.004921 |
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Target: 5'- aCACCAGAGGGAGAUCCAUGAAGAAGCu -3' miRNA: 3'- -GUGGUCUCCCUCUAGGUACUUCUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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