Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19860 | 3' | -55.8 | NC_004687.1 | + | 97747 | 0.66 | 0.916003 |
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Target: 5'- ---cGCCUC-CUUCAGGa--GGGUCUc -3' miRNA: 3'- uguaCGGGGaGAAGUCCaagCCCAGA- -5' |
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| 19860 | 3' | -55.8 | NC_004687.1 | + | 5541 | 0.66 | 0.91542 |
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Target: 5'- --cUGCUCCUCUucccagagcuugaUCAcGUUCGGGUUg -3' miRNA: 3'- uguACGGGGAGA-------------AGUcCAAGCCCAGa -5' |
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| 19860 | 3' | -55.8 | NC_004687.1 | + | 42537 | 0.66 | 0.903882 |
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Target: 5'- ---cGCaCCCagCaUCAGGUUCGGGUg- -3' miRNA: 3'- uguaCG-GGGa-GaAGUCCAAGCCCAga -5' |
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| 19860 | 3' | -55.8 | NC_004687.1 | + | 33523 | 0.67 | 0.876831 |
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Target: 5'- gACGUGCa-Cgc--CAGGUUCGGGUCg -3' miRNA: 3'- -UGUACGggGagaaGUCCAAGCCCAGa -5' |
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| 19860 | 3' | -55.8 | NC_004687.1 | + | 125871 | 0.67 | 0.846256 |
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Target: 5'- gGCcUGCUCUUCUUgCAGGUUCgccgugcgcuGGGUCc -3' miRNA: 3'- -UGuACGGGGAGAA-GUCCAAG----------CCCAGa -5' |
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| 19860 | 3' | -55.8 | NC_004687.1 | + | 57141 | 0.68 | 0.803683 |
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Target: 5'- gACA-GCa--UCUUCAGGUUCGGcGUCUu -3' miRNA: 3'- -UGUaCGgggAGAAGUCCAAGCC-CAGA- -5' |
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| 19860 | 3' | -55.8 | NC_004687.1 | + | 114676 | 0.68 | 0.793754 |
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Target: 5'- -aGUGCCCUUCUcCAGGacguaguccauguUUCGGGUg- -3' miRNA: 3'- ugUACGGGGAGAaGUCC-------------AAGCCCAga -5' |
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| 19860 | 3' | -55.8 | NC_004687.1 | + | 9560 | 0.69 | 0.776189 |
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Target: 5'- --uUGCCCUg-UUCAcGGUUCGGGUUg -3' miRNA: 3'- uguACGGGGagAAGU-CCAAGCCCAGa -5' |
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| 19860 | 3' | -55.8 | NC_004687.1 | + | 10690 | 0.71 | 0.657005 |
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Target: 5'- cGCGUGCUCCUUUgccCAGGUgucguUgGGGUCg -3' miRNA: 3'- -UGUACGGGGAGAa--GUCCA-----AgCCCAGa -5' |
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| 19860 | 3' | -55.8 | NC_004687.1 | + | 58144 | 1.07 | 0.003966 |
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Target: 5'- gACAUGCCCCUCUUCAGGUUCGGGUCUc -3' miRNA: 3'- -UGUACGGGGAGAAGUCCAAGCCCAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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