Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1987 | 3' | -56.2 | NC_001347.2 | + | 176812 | 0.66 | 0.971252 |
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Target: 5'- -cUCGUUCUCCUCCUUcuauUCccAGAUCGc -3' miRNA: 3'- gcAGCAGGAGGAGGAG----AGccUCUAGC- -5' |
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| 1987 | 3' | -56.2 | NC_001347.2 | + | 84735 | 0.66 | 0.963559 |
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Target: 5'- -aUCGUCCUCC-CCUUcguccgaggaggaagUCGGAGGg-- -3' miRNA: 3'- gcAGCAGGAGGaGGAG---------------AGCCUCUagc -5' |
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| 1987 | 3' | -56.2 | NC_001347.2 | + | 38717 | 0.66 | 0.963559 |
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Target: 5'- cCGUCG-CCUCCUCggcgcgcagcaacguCUgUCGGAGcgCc -3' miRNA: 3'- -GCAGCaGGAGGAG---------------GAgAGCCUCuaGc -5' |
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| 1987 | 3' | -56.2 | NC_001347.2 | + | 96997 | 0.66 | 0.962259 |
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Target: 5'- gGUCGUUCUCCUCgaCgcgcggcggCGGAGgAUCc -3' miRNA: 3'- gCAGCAGGAGGAGgaGa--------GCCUC-UAGc -5' |
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| 1987 | 3' | -56.2 | NC_001347.2 | + | 58972 | 0.69 | 0.895462 |
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Target: 5'- uCGUCuUCCUCCUCCUgcagUUCGGcuucGGAcUCGg -3' miRNA: 3'- -GCAGcAGGAGGAGGA----GAGCC----UCU-AGC- -5' |
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| 1987 | 3' | -56.2 | NC_001347.2 | + | 221378 | 0.69 | 0.861518 |
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Target: 5'- uGUCGUCCUCgUCCUCUUcGucGUCu -3' miRNA: 3'- gCAGCAGGAGgAGGAGAGcCucUAGc -5' |
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| 1987 | 3' | -56.2 | NC_001347.2 | + | 66633 | 0.7 | 0.838846 |
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Target: 5'- uCGUUGUCCUCCUCgUcCUCGuGGcGGUUGu -3' miRNA: 3'- -GCAGCAGGAGGAGgA-GAGC-CU-CUAGC- -5' |
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| 1987 | 3' | -56.2 | NC_001347.2 | + | 94356 | 0.71 | 0.806246 |
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Target: 5'- gCGUCGUCCUCauCUCCgCcUGGAGcgCGg -3' miRNA: 3'- -GCAGCAGGAG--GAGGaGaGCCUCuaGC- -5' |
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| 1987 | 3' | -56.2 | NC_001347.2 | + | 206546 | 0.71 | 0.797718 |
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Target: 5'- uGUUGUUuaCUCCUCCUCUUcgGGAGAcCGu -3' miRNA: 3'- gCAGCAG--GAGGAGGAGAG--CCUCUaGC- -5' |
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| 1987 | 3' | -56.2 | NC_001347.2 | + | 184452 | 0.73 | 0.67727 |
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Target: 5'- uCGUCGUCgUCCUCCUCUCccucGUCGu -3' miRNA: 3'- -GCAGCAGgAGGAGGAGAGccucUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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