Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19874 | 5' | -55.5 | NC_004687.1 | + | 73197 | 0.66 | 0.931568 |
Target: 5'- cGCGGuGAuGGCcagcuucgccaggaUCUCCUUGgccaCUUGGUc -3' miRNA: 3'- -CGCCuCU-CCG--------------AGAGGGACaa--GAACCG- -5' |
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19874 | 5' | -55.5 | NC_004687.1 | + | 15415 | 0.66 | 0.922536 |
Target: 5'- gGCGGuGAGGaUCUUcgaCCaGUUgUUGGCg -3' miRNA: 3'- -CGCCuCUCCgAGAG---GGaCAAgAACCG- -5' |
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19874 | 5' | -55.5 | NC_004687.1 | + | 106156 | 0.67 | 0.904916 |
Target: 5'- uGCGGAGGGGCa--UCCUG-UCUUuGCu -3' miRNA: 3'- -CGCCUCUCCGagaGGGACaAGAAcCG- -5' |
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19874 | 5' | -55.5 | NC_004687.1 | + | 144738 | 0.67 | 0.904916 |
Target: 5'- aCGGGGGcGGCUCUCC----UCcUGGCa -3' miRNA: 3'- cGCCUCU-CCGAGAGGgacaAGaACCG- -5' |
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19874 | 5' | -55.5 | NC_004687.1 | + | 11860 | 0.7 | 0.75004 |
Target: 5'- uGCGGGaaauuGAGGCUCUCCggGUccgaccgaUUGGCg -3' miRNA: 3'- -CGCCU-----CUCCGAGAGGgaCAag------AACCG- -5' |
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19874 | 5' | -55.5 | NC_004687.1 | + | 49797 | 1.12 | 0.001942 |
Target: 5'- uGCGGAGAGGCUCUCCCUGUUCUUGGCc -3' miRNA: 3'- -CGCCUCUCCGAGAGGGACAAGAACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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