miRNA: 3'- -AGUACUACGGCUGaa-CA-----CCGAUCacu -5'
miRNA: 3'- -AGUACUACGGcugaACACCgA-UCACU- -5'
miRNA: 3'- -AGU--ACUaCGGC-UGAac--ACCGAUCACU- -5'
miRNA: 3'- aGUACUA-CgGCUGaa-CACCGa--UCACU- -5'
miRNA: 3'- -AGUACUACGGCUGAACACCGAUCACU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19900 | 5' | -50.7 | NC_004687.1 | + | 90995 | 0.66 | 0.993636 |
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Target: 5'- aUCAUGGUGCCGGUgggGUugaucGGCUGGg-- -3' miRNA: 3'- -AGUACUACGGCUGaa-CA-----CCGAUCacu -5' |
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| 19900 | 5' | -50.7 | NC_004687.1 | + | 132552 | 0.66 | 0.993636 |
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Target: 5'- gUCAUGGUGCUccugUGUGGgUgAGUGAa -3' miRNA: 3'- -AGUACUACGGcugaACACCgA-UCACU- -5' |
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| 19900 | 5' | -50.7 | NC_004687.1 | + | 70981 | 0.66 | 0.992645 |
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Target: 5'- gUCGgcUGAgGCCGcGCUggucUGGCUGGUGGa -3' miRNA: 3'- -AGU--ACUaCGGC-UGAac--ACCGAUCACU- -5' |
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| 19900 | 5' | -50.7 | NC_004687.1 | + | 73964 | 0.67 | 0.981847 |
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Target: 5'- gCAUGGUcGgCGGCgcgGUGGCcggAGUGAc -3' miRNA: 3'- aGUACUA-CgGCUGaa-CACCGa--UCACU- -5' |
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| 19900 | 5' | -50.7 | NC_004687.1 | + | 32402 | 1.1 | 0.007544 |
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Target: 5'- gUCAUGAUGCCGACUUGUGGCUAGUGAc -3' miRNA: 3'- -AGUACUACGGCUGAACACCGAUCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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