Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19905 | 5' | -52.2 | NC_004687.1 | + | 68227 | 0.66 | 0.975496 |
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Target: 5'- aGggUCGGGGCUCcg----GUCgaACUCa -3' miRNA: 3'- aCuuGGCCCCGAGaacauaCAG--UGAG- -5' |
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| 19905 | 5' | -52.2 | NC_004687.1 | + | 57036 | 0.67 | 0.963353 |
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Target: 5'- cGGGCUGGGcGCgCUUGUuggccAUGUCGC-Ca -3' miRNA: 3'- aCUUGGCCC-CGaGAACA-----UACAGUGaG- -5' |
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| 19905 | 5' | -52.2 | NC_004687.1 | + | 97143 | 0.67 | 0.959758 |
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Target: 5'- cGGGCCGGGgaGCUCcgG-AUGUCcCUCg -3' miRNA: 3'- aCUUGGCCC--CGAGaaCaUACAGuGAG- -5' |
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| 19905 | 5' | -52.2 | NC_004687.1 | + | 71338 | 0.68 | 0.947536 |
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Target: 5'- -uGGCCGGGGCUCagGUGguUCAgUCc -3' miRNA: 3'- acUUGGCCCCGAGaaCAUacAGUgAG- -5' |
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| 19905 | 5' | -52.2 | NC_004687.1 | + | 50887 | 0.68 | 0.938154 |
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Target: 5'- cGu-CCGGaGGCgcgggCUUGUAgaaGUCGCUCu -3' miRNA: 3'- aCuuGGCC-CCGa----GAACAUa--CAGUGAG- -5' |
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| 19905 | 5' | -52.2 | NC_004687.1 | + | 66675 | 0.69 | 0.915152 |
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Target: 5'- cUGGGCCGGGGCUCgauccugGUCGg-- -3' miRNA: 3'- -ACUUGGCCCCGAGaacaua-CAGUgag -5' |
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| 19905 | 5' | -52.2 | NC_004687.1 | + | 96050 | 0.71 | 0.832777 |
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Target: 5'- gUGGGCCaGGGCUCUUcGUAUGccugguauugaggCGCUCc -3' miRNA: 3'- -ACUUGGcCCCGAGAA-CAUACa------------GUGAG- -5' |
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| 19905 | 5' | -52.2 | NC_004687.1 | + | 127043 | 0.76 | 0.586218 |
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Target: 5'- cUGGACCGGGGCUCUUGcGU-UUGCUa -3' miRNA: 3'- -ACUUGGCCCCGAGAACaUAcAGUGAg -5' |
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| 19905 | 5' | -52.2 | NC_004687.1 | + | 61256 | 0.76 | 0.575751 |
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Target: 5'- aGAacuuGCCGGGGCUCUgaggGUuUGUCACc- -3' miRNA: 3'- aCU----UGGCCCCGAGAa---CAuACAGUGag -5' |
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| 19905 | 5' | -52.2 | NC_004687.1 | + | 28910 | 1.11 | 0.004596 |
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Target: 5'- uUGAACCGGGGCUCUUGUAUGUCACUCg -3' miRNA: 3'- -ACUUGGCCCCGAGAACAUACAGUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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