Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1999 | 3' | -60 | NC_001347.2 | + | 185335 | 0.66 | 0.878568 |
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Target: 5'- aUAGUauaGGuCCGUGcGAGcAGGCcGcCGCCg -3' miRNA: 3'- gGUCAg--CC-GGCAC-CUC-UCCG-CaGCGG- -5' |
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| 1999 | 3' | -60 | NC_001347.2 | + | 174644 | 0.66 | 0.881208 |
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Target: 5'- gCGGUCGGucuacgucuacucgcCCGUGGuGGAaucgcuguaccuGGUGUCGCg -3' miRNA: 3'- gGUCAGCC---------------GGCACC-UCU------------CCGCAGCGg -5' |
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| 1999 | 3' | -60 | NC_001347.2 | + | 182190 | 0.66 | 0.885111 |
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Target: 5'- -gGGcCGGCCcucggggugcggGUGGcGGGcuGCGUCGCCu -3' miRNA: 3'- ggUCaGCCGG------------CACCuCUC--CGCAGCGG- -5' |
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| 1999 | 3' | -60 | NC_001347.2 | + | 228594 | 0.66 | 0.885111 |
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Target: 5'- uCCAGUCagcgaGGCCGUcGcGuuGGCG-CGCCa -3' miRNA: 3'- -GGUCAG-----CCGGCAcCuCu-CCGCaGCGG- -5' |
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| 1999 | 3' | -60 | NC_001347.2 | + | 153247 | 0.66 | 0.885111 |
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Target: 5'- --cGUCGGCCGgc--GAcGGCGguUCGCCg -3' miRNA: 3'- gguCAGCCGGCaccuCU-CCGC--AGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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