Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1999 | 3' | -60 | NC_001347.2 | + | 225629 | 0.66 | 0.871835 |
Target: 5'- aCAG-CaGUCGUGGA-AGG-GUCGCCa -3' miRNA: 3'- gGUCaGcCGGCACCUcUCCgCAGCGG- -5' |
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1999 | 3' | -60 | NC_001347.2 | + | 162169 | 0.66 | 0.864915 |
Target: 5'- -aAGUCGGCgG-GcGGGGGCGUgauccgaccCGCCa -3' miRNA: 3'- ggUCAGCCGgCaCcUCUCCGCA---------GCGG- -5' |
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1999 | 3' | -60 | NC_001347.2 | + | 684 | 0.66 | 0.871835 |
Target: 5'- cCCAGUccaCGGCCuuccagacugcGUGGcGccaAGGCGgCGCCa -3' miRNA: 3'- -GGUCA---GCCGG-----------CACCuC---UCCGCaGCGG- -5' |
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1999 | 3' | -60 | NC_001347.2 | + | 75858 | 0.66 | 0.857813 |
Target: 5'- --cGUCGGCCGc---GGGGCGcagCGCCu -3' miRNA: 3'- gguCAGCCGGCaccuCUCCGCa--GCGG- -5' |
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1999 | 3' | -60 | NC_001347.2 | + | 222404 | 1.14 | 0.001204 |
Target: 5'- aCCAGUCGGCCGUGGAGAGGCGUCGCCg -3' miRNA: 3'- -GGUCAGCCGGCACCUCUCCGCAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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