Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2004 | 5' | -58.4 | NC_001347.2 | + | 155350 | 0.66 | 0.898834 |
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Target: 5'- gGGCCCGuGAGggGGAgucGUUGGGcCGUUa- -3' miRNA: 3'- -CCGGGC-CUUa-CCUa--CAGCCC-GCAGgu -5' |
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| 2004 | 5' | -58.4 | NC_001347.2 | + | 77319 | 0.67 | 0.85103 |
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Target: 5'- gGGCCgCGGcGUGGAUGaCGaGGcCGUCa- -3' miRNA: 3'- -CCGG-GCCuUACCUACaGC-CC-GCAGgu -5' |
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| 2004 | 5' | -58.4 | NC_001347.2 | + | 31667 | 0.67 | 0.843445 |
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Target: 5'- uGGCUCGGGucGGcugGUCGGGcCGUCgCGg -3' miRNA: 3'- -CCGGGCCUuaCCua-CAGCCC-GCAG-GU- -5' |
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| 2004 | 5' | -58.4 | NC_001347.2 | + | 135920 | 0.7 | 0.739922 |
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Target: 5'- gGGCCaCGGAcgGGAaccgggaccccggUG-CGGGgGUCCc -3' miRNA: 3'- -CCGG-GCCUuaCCU-------------ACaGCCCgCAGGu -5' |
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| 2004 | 5' | -58.4 | NC_001347.2 | + | 139336 | 0.71 | 0.664783 |
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Target: 5'- uGCgCGGcGGUGGGcUGUCaGGCGUCCGa -3' miRNA: 3'- cCGgGCC-UUACCU-ACAGcCCGCAGGU- -5' |
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| 2004 | 5' | -58.4 | NC_001347.2 | + | 208618 | 0.73 | 0.548829 |
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Target: 5'- -aCCUGGAG-GGA--UCGGGCGUCCAc -3' miRNA: 3'- ccGGGCCUUaCCUacAGCCCGCAGGU- -5' |
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| 2004 | 5' | -58.4 | NC_001347.2 | + | 573 | 1.1 | 0.002567 |
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Target: 5'- cGGCCCGGAAUGGAUGUCGGGCGUCCAc -3' miRNA: 3'- -CCGGGCCUUACCUACAGCCCGCAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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