Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2013 | 3' | -52.1 | NC_001347.2 | + | 159830 | 0.66 | 0.992652 |
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Target: 5'- cGGCgUUUCGgUcCACgUGUCCUGGAGc -3' miRNA: 3'- uCCG-AAAGCgGuGUGgAUAGGACUUU- -5' |
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| 2013 | 3' | -52.1 | NC_001347.2 | + | 79147 | 0.66 | 0.990362 |
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Target: 5'- aAGGUccgCGCCGCGCCg--CUUGAu- -3' miRNA: 3'- -UCCGaaaGCGGUGUGGauaGGACUuu -5' |
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| 2013 | 3' | -52.1 | NC_001347.2 | + | 172173 | 0.67 | 0.982183 |
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Target: 5'- uGGGCUUcgCGCgGCACCgUGUUCgagGAGGa -3' miRNA: 3'- -UCCGAAa-GCGgUGUGG-AUAGGa--CUUU- -5' |
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| 2013 | 3' | -52.1 | NC_001347.2 | + | 14865 | 0.67 | 0.982183 |
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Target: 5'- aGGGCUcguaCGCCACGgCCgaacgaAUCCUGGGu -3' miRNA: 3'- -UCCGAaa--GCGGUGU-GGa-----UAGGACUUu -5' |
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| 2013 | 3' | -52.1 | NC_001347.2 | + | 88063 | 0.67 | 0.977746 |
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Target: 5'- cGGGCccgaUGCCACgGCgCUGUCCUGAc- -3' miRNA: 3'- -UCCGaaa-GCGGUG-UG-GAUAGGACUuu -5' |
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| 2013 | 3' | -52.1 | NC_001347.2 | + | 87267 | 0.67 | 0.977746 |
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Target: 5'- cGGCg--CGCCGUACCcgucUCCUGAAAg -3' miRNA: 3'- uCCGaaaGCGGUGUGGau--AGGACUUU- -5' |
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| 2013 | 3' | -52.1 | NC_001347.2 | + | 112118 | 0.68 | 0.966515 |
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Target: 5'- uGGCgUUCaCCACGCCgg-CCUGAGc -3' miRNA: 3'- uCCGaAAGcGGUGUGGauaGGACUUu -5' |
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| 2013 | 3' | -52.1 | NC_001347.2 | + | 41336 | 0.69 | 0.959608 |
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Target: 5'- cAGGCUUUCGCgCACACgauUCCgagGAc- -3' miRNA: 3'- -UCCGAAAGCG-GUGUGgauAGGa--CUuu -5' |
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| 2013 | 3' | -52.1 | NC_001347.2 | + | 208811 | 0.71 | 0.897964 |
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Target: 5'- gAGGCUcagccgcugCGCCGCGCCgccGUCCUcGAAGc -3' miRNA: 3'- -UCCGAaa-------GCGGUGUGGa--UAGGA-CUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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