miRNA: 3'- acUGGUCCaaCUCUGGCG----AGuAAAGc -5'
miRNA: 3'- acUGguCCa---ACUCUGGCGAGUaaagc -5'
miRNA: 3'- -ACUGGUC-CaacUCUGGCGAGUAAAgC- -5'
miRNA: 3'- acUGGUC-CAACUCUGGCGAGuaaaGC- -5'
miRNA: 3'- aCUGGUCCa---ACUcUGGCGAGUaa---AGC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2030 | 5' | -51 | NC_001347.2 | + | 67114 | 0.67 | 0.996334 |
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Target: 5'- -aACCAGGggGAGAUCGCacaaUCuUUUCu -3' miRNA: 3'- acUGGUCCaaCUCUGGCG----AGuAAAGc -5' |
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| 2030 | 5' | -51 | NC_001347.2 | + | 188639 | 0.67 | 0.995024 |
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Target: 5'- -cGCguGGgacgUGAGGCCGCUCAc---- -3' miRNA: 3'- acUGguCCa---ACUCUGGCGAGUaaagc -5' |
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| 2030 | 5' | -51 | NC_001347.2 | + | 111113 | 0.68 | 0.992369 |
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Target: 5'- cUGACCGGcGaucAGGCCGUUCAUUUgGa -3' miRNA: 3'- -ACUGGUC-CaacUCUGGCGAGUAAAgC- -5' |
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| 2030 | 5' | -51 | NC_001347.2 | + | 210020 | 0.72 | 0.93325 |
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Target: 5'- -cGCCGGuGUUGGGACCGCUUcccgCGg -3' miRNA: 3'- acUGGUC-CAACUCUGGCGAGuaaaGC- -5' |
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| 2030 | 5' | -51 | NC_001347.2 | + | 14018 | 0.72 | 0.922639 |
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Target: 5'- cGGCCGGGgcugUGA-ACCGCUCAguggcUCGg -3' miRNA: 3'- aCUGGUCCa---ACUcUGGCGAGUaa---AGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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