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miRNA: 3'- -CAGaGGGUUCCUUGCgCCa-------GCUGCUG- -5'
miRNA: 3'- -CAGAG-GGUUCCuu--GCG-------------CCA--GCUGCUG- -5'
miRNA: 3'- caGAGGGU----UCC---UUGCGCCaGC--UGCUG- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 2031 | 3' | -55.7 | NC_001347.2 | + | 66565 | 0.66 | 0.960904 |
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Target: 5'- cGUC-CUCGAGGAACGaGGgcccucgcCGGCGGCc -3' miRNA: 3'- -CAGaGGGUUCCUUGCgCCa-------GCUGCUG- -5' |
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| 2031 | 3' | -55.7 | NC_001347.2 | + | 128244 | 0.66 | 0.957015 |
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Target: 5'- gGUCUCaCCGAGGugcuCGCcuaucaccuguacGGUggCGACGGCg -3' miRNA: 3'- -CAGAG-GGUUCCuu--GCG-------------CCA--GCUGCUG- -5' |
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| 2031 | 3' | -55.7 | NC_001347.2 | + | 36905 | 0.66 | 0.970257 |
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Target: 5'- -aUUCCCAucuuGGGcgcGACGCGGaCGcaGCGACa -3' miRNA: 3'- caGAGGGU----UCC---UUGCGCCaGC--UGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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