Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2031 | 3' | -55.7 | NC_001347.2 | + | 5672 | 0.66 | 0.964222 |
Target: 5'- uUCgCCCGAGGAagACGC---CGACGACu -3' miRNA: 3'- cAGaGGGUUCCU--UGCGccaGCUGCUG- -5' |
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2031 | 3' | -55.7 | NC_001347.2 | + | 173468 | 0.66 | 0.967338 |
Target: 5'- uUCUCaCCGAGGAaccuuuccaGCGUGG-CGAucccuuCGACa -3' miRNA: 3'- cAGAG-GGUUCCU---------UGCGCCaGCU------GCUG- -5' |
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2031 | 3' | -55.7 | NC_001347.2 | + | 36905 | 0.66 | 0.970257 |
Target: 5'- -aUUCCCAucuuGGGcgcGACGCGGaCGcaGCGACa -3' miRNA: 3'- caGAGGGU----UCC---UUGCGCCaGC--UGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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