miRNA: 3'- cAGAGG-GuuCCUUGCGCCa-GCugCUG- -5'
miRNA: 3'- cagagGGUUCCUUGCgCCa---GCUGCUG- -5'
miRNA: 3'- cagAGGGuuCCUUGCgCCAGCUGCUG- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2031 | 3' | -55.7 | NC_001347.2 | + | 94367 | 0.71 | 0.763657 |
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Target: 5'- aUCUCCgCcuGGAGCGCGGccCGugGAUc -3' miRNA: 3'- cAGAGG-GuuCCUUGCGCCa-GCugCUG- -5' |
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| 2031 | 3' | -55.7 | NC_001347.2 | + | 39509 | 0.72 | 0.735594 |
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Target: 5'- ----aCCAAGGGGCGgGGggcgCGGCGACa -3' miRNA: 3'- cagagGGUUCCUUGCgCCa---GCUGCUG- -5' |
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| 2031 | 3' | -55.7 | NC_001347.2 | + | 225899 | 0.78 | 0.429259 |
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Target: 5'- --gUCCUggGGAACG-GGUCGGCGGCc -3' miRNA: 3'- cagAGGGuuCCUUGCgCCAGCUGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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