Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2038 | 3' | -53 | NC_001347.2 | + | 78341 | 0.72 | 0.882355 |
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Target: 5'- cGCGCguggUCCGGGCGgaccCGGCGUgGUg- -3' miRNA: 3'- -CGUGa---AGGUCUGCaa--GCCGCAgCAgg -5' |
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| 2038 | 3' | -53 | NC_001347.2 | + | 168093 | 0.74 | 0.821352 |
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Target: 5'- aGCACcgugUCCAGuuCGgugUCGGUGUCGcgCCg -3' miRNA: 3'- -CGUGa---AGGUCu-GCa--AGCCGCAGCa-GG- -5' |
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| 2038 | 3' | -53 | NC_001347.2 | + | 181181 | 0.75 | 0.73765 |
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Target: 5'- cGUACg-CCAGuACGUcuugguggacacuuUCGGCGUCGUCUa -3' miRNA: 3'- -CGUGaaGGUC-UGCA--------------AGCCGCAGCAGG- -5' |
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| 2038 | 3' | -53 | NC_001347.2 | + | 142422 | 0.76 | 0.711631 |
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Target: 5'- cGCGC--CCAGugGcgUCGGCGcggCGUCCg -3' miRNA: 3'- -CGUGaaGGUCugCa-AGCCGCa--GCAGG- -5' |
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| 2038 | 3' | -53 | NC_001347.2 | + | 94310 | 0.76 | 0.701866 |
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Target: 5'- aGCGCgaucgccgcCCAcGGCGUccUCGGCGUCGUCg -3' miRNA: 3'- -CGUGaa-------GGU-CUGCA--AGCCGCAGCAGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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