Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2038 | 3' | -53 | NC_001347.2 | + | 188464 | 0.66 | 0.992027 |
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Target: 5'- gGCGCcgCUAGugGUggCGGUGUU-UCCa -3' miRNA: 3'- -CGUGaaGGUCugCAa-GCCGCAGcAGG- -5' |
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| 2038 | 3' | -53 | NC_001347.2 | + | 193085 | 0.66 | 0.995419 |
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Target: 5'- gGCAUgUCCGGcCGUcgcaagggcugcUCGGCGgccacggCGUCUu -3' miRNA: 3'- -CGUGaAGGUCuGCA------------AGCCGCa------GCAGG- -5' |
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| 2038 | 3' | -53 | NC_001347.2 | + | 193389 | 0.66 | 0.995419 |
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Target: 5'- aGCACUgCCAGaACGacuuuUUCGGCGaauUCCg -3' miRNA: 3'- -CGUGAaGGUC-UGC-----AAGCCGCagcAGG- -5' |
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| 2038 | 3' | -53 | NC_001347.2 | + | 196967 | 0.66 | 0.993911 |
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Target: 5'- cGCGCcgCC-GACGcccgagaCGGCGcgCGUCCa -3' miRNA: 3'- -CGUGaaGGuCUGCaa-----GCCGCa-GCAGG- -5' |
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| 2038 | 3' | -53 | NC_001347.2 | + | 220409 | 0.66 | 0.992027 |
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Target: 5'- aCGCUUgugaggugaCAGAUGUgugUGGCGUCGUUg -3' miRNA: 3'- cGUGAAg--------GUCUGCAa--GCCGCAGCAGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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