miRNA: 3'- -CGUGa---AGGUCUGCaa--GCCGCAgCAgg -5'
miRNA: 3'- -CgUGAAGgUCUGC----AAGCCGCaGCa--GG- -5'
miRNA: 3'- -CGUGAAGguCugcaaGCCGCaGCAGG- -5'
miRNA: 3'- -CGUGaaGGUCUGCaaGCCGCAgCagg -5'
miRNA: 3'- -CGUGaAGGUCugCAaGC----------CGCAGcAGG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2038 | 3' | -53 | NC_001347.2 | + | 78341 | 0.72 | 0.882355 |
|
Target: 5'- cGCGCguggUCCGGGCGgaccCGGCGUgGUg- -3' miRNA: 3'- -CGUGa---AGGUCUGCaa--GCCGCAgCAgg -5' |
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| 2038 | 3' | -53 | NC_001347.2 | + | 40514 | 0.68 | 0.979525 |
|
Target: 5'- cGgGCUUCgGGACGguguUUCGGCG-CGcugCCg -3' miRNA: 3'- -CgUGAAGgUCUGC----AAGCCGCaGCa--GG- -5' |
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| 2038 | 3' | -53 | NC_001347.2 | + | 17612 | 0.67 | 0.989708 |
|
Target: 5'- aGCACUUUguGuagg-CGGCG-CGUCCc -3' miRNA: 3'- -CGUGAAGguCugcaaGCCGCaGCAGG- -5' |
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| 2038 | 3' | -53 | NC_001347.2 | + | 1324 | 0.67 | 0.985279 |
|
Target: 5'- cGCGCagCCAGAUGaaCGGCGUgGg-- -3' miRNA: 3'- -CGUGaaGGUCUGCaaGCCGCAgCagg -5' |
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| 2038 | 3' | -53 | NC_001347.2 | + | 631 | 0.66 | 0.994709 |
|
Target: 5'- uGCGC-UCCAGugGUaCGcgccugccgcGCGUCuUCCu -3' miRNA: 3'- -CGUGaAGGUCugCAaGC----------CGCAGcAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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