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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2040 | 3' | -56.9 | NC_001347.2 | + | 150865 | 0.66 | 0.94406 |
Target: 5'- uGCgUGCUUCGGUgcgcgUCUCGGUGCucucGGAa -3' miRNA: 3'- -CG-ACGGAGUCAg----AGAGCCACGca--CCU- -5' |
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2040 | 3' | -56.9 | NC_001347.2 | + | 94996 | 0.66 | 0.930038 |
Target: 5'- cGCUGCC-CAGaUCacacaGGUGCGUGcGAa -3' miRNA: 3'- -CGACGGaGUC-AGagag-CCACGCAC-CU- -5' |
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2040 | 3' | -56.9 | NC_001347.2 | + | 201479 | 0.68 | 0.882878 |
Target: 5'- cGCUGCCUCGuuauccggcGUCgcuggcgCUUGGUGCGcgacgaaGGAc -3' miRNA: 3'- -CGACGGAGU---------CAGa------GAGCCACGCa------CCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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