Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20405 | 3' | -54.8 | NC_004688.1 | + | 26038 | 0.66 | 0.908058 |
Target: 5'- gGCUcCGGgGAggCCCgUGGGcauaccgguGCUGAGCg -3' miRNA: 3'- -CGGuGCCgCUuaGGG-AUCC---------CGAUUCG- -5' |
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20405 | 3' | -54.8 | NC_004688.1 | + | 26991 | 0.66 | 0.903601 |
Target: 5'- gGCCACGGCGccaguccgccguugaGAUagaagaugaucucCCCgccGGGGCUGGuGUa -3' miRNA: 3'- -CGGUGCCGC---------------UUA-------------GGGa--UCCCGAUU-CG- -5' |
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20405 | 3' | -54.8 | NC_004688.1 | + | 48099 | 0.66 | 0.901653 |
Target: 5'- gGCCGCGGUaGAGgcggccaaCCCUAacuGGCggGAGCa -3' miRNA: 3'- -CGGUGCCG-CUUa-------GGGAUc--CCGa-UUCG- -5' |
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20405 | 3' | -54.8 | NC_004688.1 | + | 60713 | 0.66 | 0.895002 |
Target: 5'- uGCCGCuguaGGCGAcgguGUCCCagAGGuGCcucuGGCg -3' miRNA: 3'- -CGGUG----CCGCU----UAGGGa-UCC-CGau--UCG- -5' |
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20405 | 3' | -54.8 | NC_004688.1 | + | 67294 | 0.66 | 0.895002 |
Target: 5'- --gACGGUGAacucgaAUUCCgAGaGGCUGAGCu -3' miRNA: 3'- cggUGCCGCU------UAGGGaUC-CCGAUUCG- -5' |
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20405 | 3' | -54.8 | NC_004688.1 | + | 78880 | 0.66 | 0.890894 |
Target: 5'- aGCCACGGgGAGcCCCaggacGGaguccugcagccccaGCUGAGCc -3' miRNA: 3'- -CGGUGCCgCUUaGGGau---CC---------------CGAUUCG- -5' |
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20405 | 3' | -54.8 | NC_004688.1 | + | 25675 | 0.66 | 0.880249 |
Target: 5'- cGCCACGaucgccuGCGcuUCCUgcGGGCUAuacccGCg -3' miRNA: 3'- -CGGUGC-------CGCuuAGGGauCCCGAUu----CG- -5' |
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20405 | 3' | -54.8 | NC_004688.1 | + | 57945 | 0.66 | 0.873608 |
Target: 5'- aCCGCGGCccgGGGgcaCCUcGGGGCUGcuGGCc -3' miRNA: 3'- cGGUGCCG---CUUag-GGA-UCCCGAU--UCG- -5' |
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20405 | 3' | -54.8 | NC_004688.1 | + | 52738 | 0.66 | 0.872107 |
Target: 5'- cGCCGCGccgauccGCGAAUgCUgGGGGCcggacuccccgcaUGAGCa -3' miRNA: 3'- -CGGUGC-------CGCUUAgGGaUCCCG-------------AUUCG- -5' |
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20405 | 3' | -54.8 | NC_004688.1 | + | 4205 | 0.67 | 0.866012 |
Target: 5'- cGCCGCGGgcgcCGAggCCCUcgguuAGGGCcauuGGUu -3' miRNA: 3'- -CGGUGCC----GCUuaGGGA-----UCCCGau--UCG- -5' |
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20405 | 3' | -54.8 | NC_004688.1 | + | 1024 | 0.67 | 0.866012 |
Target: 5'- cGCCcCcGCGAGUCaa-AGGGCUAAGa -3' miRNA: 3'- -CGGuGcCGCUUAGggaUCCCGAUUCg -5' |
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20405 | 3' | -54.8 | NC_004688.1 | + | 92952 | 0.67 | 0.850158 |
Target: 5'- gGCCGCGGCGAuUCUgCUucggagucgGGGGCguuacccccAGCu -3' miRNA: 3'- -CGGUGCCGCUuAGG-GA---------UCCCGau-------UCG- -5' |
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20405 | 3' | -54.8 | NC_004688.1 | + | 44991 | 0.67 | 0.833468 |
Target: 5'- aGCCACGGCagcauuccGAAccaCCCgguGGGCUcguaugccgGGGCg -3' miRNA: 3'- -CGGUGCCG--------CUUa--GGGau-CCCGA---------UUCG- -5' |
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20405 | 3' | -54.8 | NC_004688.1 | + | 70492 | 0.68 | 0.816003 |
Target: 5'- cGCC-CGGUGAuacUCCCacGGGGagGAGCa -3' miRNA: 3'- -CGGuGCCGCUu--AGGGa-UCCCgaUUCG- -5' |
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20405 | 3' | -54.8 | NC_004688.1 | + | 42878 | 0.68 | 0.807001 |
Target: 5'- aCCuCGuaGAggCCCUcguagccgGGGGCUGGGCg -3' miRNA: 3'- cGGuGCcgCUuaGGGA--------UCCCGAUUCG- -5' |
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20405 | 3' | -54.8 | NC_004688.1 | + | 99355 | 0.68 | 0.807001 |
Target: 5'- aGCCG-GGUG-AUCCUggacgAGGGCgGAGCc -3' miRNA: 3'- -CGGUgCCGCuUAGGGa----UCCCGaUUCG- -5' |
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20405 | 3' | -54.8 | NC_004688.1 | + | 97903 | 0.68 | 0.797832 |
Target: 5'- cGCCcCGcGCGGGcCUCUcGGcGGCUAGGCg -3' miRNA: 3'- -CGGuGC-CGCUUaGGGA-UC-CCGAUUCG- -5' |
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20405 | 3' | -54.8 | NC_004688.1 | + | 86576 | 0.69 | 0.76941 |
Target: 5'- aGCCACGGCcAGcUCCauuguuaUGGGGCgguAGUa -3' miRNA: 3'- -CGGUGCCGcUU-AGGg------AUCCCGau-UCG- -5' |
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20405 | 3' | -54.8 | NC_004688.1 | + | 93300 | 0.69 | 0.768441 |
Target: 5'- cGCCAUGGCcggGAGUCCaugacccUUGGGGgUGAuGCg -3' miRNA: 3'- -CGGUGCCG---CUUAGG-------GAUCCCgAUU-CG- -5' |
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20405 | 3' | -54.8 | NC_004688.1 | + | 61831 | 0.69 | 0.749798 |
Target: 5'- gGCUGCGGCGGuaguUgCCgcGGGGCUcgucgGAGCu -3' miRNA: 3'- -CGGUGCCGCUu---AgGGa-UCCCGA-----UUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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