Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20406 | 5' | -55.7 | NC_004688.1 | + | 62886 | 0.66 | 0.882026 |
Target: 5'- aGGCgGguGGGCGUcGGAGagCGCCCa -3' miRNA: 3'- gCCGgCauCCCGUA-CUUCaaGUGGGc -5' |
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20406 | 5' | -55.7 | NC_004688.1 | + | 70520 | 0.66 | 0.874761 |
Target: 5'- aGGUCagGGGGCGgccGAAGUcguccagCGCCCGg -3' miRNA: 3'- gCCGGcaUCCCGUa--CUUCAa------GUGGGC- -5' |
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20406 | 5' | -55.7 | NC_004688.1 | + | 66457 | 0.66 | 0.851637 |
Target: 5'- uCGGCCGUAGaGuuccaacgccGCGUGGAGggaUguCCCGa -3' miRNA: 3'- -GCCGGCAUC-C----------CGUACUUCa--AguGGGC- -5' |
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20406 | 5' | -55.7 | NC_004688.1 | + | 6579 | 0.66 | 0.851637 |
Target: 5'- gCGGCCGUcGGuGCAgcgcugGGAGcccacgaUCGCCCa -3' miRNA: 3'- -GCCGGCAuCC-CGUa-----CUUCa------AGUGGGc -5' |
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20406 | 5' | -55.7 | NC_004688.1 | + | 33955 | 0.66 | 0.843507 |
Target: 5'- aGGCagcggGUcGGGCcgGAAGaaCACCCa -3' miRNA: 3'- gCCGg----CAuCCCGuaCUUCaaGUGGGc -5' |
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20406 | 5' | -55.7 | NC_004688.1 | + | 29707 | 0.68 | 0.781464 |
Target: 5'- gGGUCGUugcccGGC-UGggGaUCACCCGg -3' miRNA: 3'- gCCGGCAuc---CCGuACuuCaAGUGGGC- -5' |
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20406 | 5' | -55.7 | NC_004688.1 | + | 43277 | 0.68 | 0.781464 |
Target: 5'- cCGGCCGccaucgGGGGCgauGUGAGuGUUUugCUGa -3' miRNA: 3'- -GCCGGCa-----UCCCG---UACUU-CAAGugGGC- -5' |
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20406 | 5' | -55.7 | NC_004688.1 | + | 48557 | 0.69 | 0.722755 |
Target: 5'- gGGcCCGUGGGGgaaCGUcGAGGUguucCGCCCGu -3' miRNA: 3'- gCC-GGCAUCCC---GUA-CUUCAa---GUGGGC- -5' |
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20406 | 5' | -55.7 | NC_004688.1 | + | 48343 | 0.69 | 0.71263 |
Target: 5'- uGGCCcaGUGGGGCuacagggugGUGGAGcagcucUUCACCCc -3' miRNA: 3'- gCCGG--CAUCCCG---------UACUUC------AAGUGGGc -5' |
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20406 | 5' | -55.7 | NC_004688.1 | + | 96133 | 0.7 | 0.629862 |
Target: 5'- gCGG-CGcAGGGCAUGAAGUagaagUCACCa- -3' miRNA: 3'- -GCCgGCaUCCCGUACUUCA-----AGUGGgc -5' |
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20406 | 5' | -55.7 | NC_004688.1 | + | 67586 | 0.72 | 0.516906 |
Target: 5'- gCGGUCGgcGGGC-UGAGGUcCACCUu -3' miRNA: 3'- -GCCGGCauCCCGuACUUCAaGUGGGc -5' |
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20406 | 5' | -55.7 | NC_004688.1 | + | 17020 | 0.73 | 0.477706 |
Target: 5'- gGGCuugCGUGGGGCAUGGauGGUgcaGCCCa -3' miRNA: 3'- gCCG---GCAUCCCGUACU--UCAag-UGGGc -5' |
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20406 | 5' | -55.7 | NC_004688.1 | + | 93479 | 0.73 | 0.477706 |
Target: 5'- gCGGCC---GGGCGUGAGGUcgCACCaCGg -3' miRNA: 3'- -GCCGGcauCCCGUACUUCAa-GUGG-GC- -5' |
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20406 | 5' | -55.7 | NC_004688.1 | + | 30361 | 0.75 | 0.395415 |
Target: 5'- gCGGCCGaucucgUAGGGCAggccguuuuUGAAGgugaccUCGCCCGa -3' miRNA: 3'- -GCCGGC------AUCCCGU---------ACUUCa-----AGUGGGC- -5' |
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20406 | 5' | -55.7 | NC_004688.1 | + | 1940 | 1.1 | 0.001994 |
Target: 5'- gCGGCCGUAGGGCAUGAAGUUCACCCGg -3' miRNA: 3'- -GCCGGCAUCCCGUACUUCAAGUGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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