Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20407 | 3' | -57.6 | NC_004688.1 | + | 6403 | 0.66 | 0.769076 |
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Target: 5'- cGAGgcGGuCCAccccgaAGUcGCGGaGGUUGGCc -3' miRNA: 3'- uCUCuaCC-GGU------UCA-CGCC-CCAACCGc -5' |
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| 20407 | 3' | -57.6 | NC_004688.1 | + | 9058 | 0.67 | 0.729886 |
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Target: 5'- aAGGGAgcgcaGGCCGacgcuGGUGgucGGGUUGGCGc -3' miRNA: 3'- -UCUCUa----CCGGU-----UCACgc-CCCAACCGC- -5' |
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| 20407 | 3' | -57.6 | NC_004688.1 | + | 77941 | 0.68 | 0.668672 |
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Target: 5'- -uGGGUGGCCGGGcgGCGGGa-UGGUa -3' miRNA: 3'- ucUCUACCGGUUCa-CGCCCcaACCGc -5' |
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| 20407 | 3' | -57.6 | NC_004688.1 | + | 39773 | 0.68 | 0.627121 |
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Target: 5'- cGGGGGUGag-GAGUGCGaGGGUgagGGCGg -3' miRNA: 3'- -UCUCUACcggUUCACGC-CCCAa--CCGC- -5' |
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| 20407 | 3' | -57.6 | NC_004688.1 | + | 31019 | 0.69 | 0.575325 |
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Target: 5'- cGAGGUuGCCAGGUcGuCGGcGUUGGCGa -3' miRNA: 3'- uCUCUAcCGGUUCA-C-GCCcCAACCGC- -5' |
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| 20407 | 3' | -57.6 | NC_004688.1 | + | 54471 | 0.71 | 0.489081 |
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Target: 5'- aGGAGAUGGCCAgcgaggcgcAggugugccggaggucGUGCGGGGUgauccgGGgGa -3' miRNA: 3'- -UCUCUACCGGU---------U---------------CACGCCCCAa-----CCgC- -5' |
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| 20407 | 3' | -57.6 | NC_004688.1 | + | 39697 | 0.76 | 0.235579 |
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Target: 5'- uGGGGGUgGGCCGuccgGCGGGGUUGGUu -3' miRNA: 3'- -UCUCUA-CCGGUuca-CGCCCCAACCGc -5' |
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| 20407 | 3' | -57.6 | NC_004688.1 | + | 2175 | 1.08 | 0.001607 |
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Target: 5'- aAGAGAUGGCCAAGUGCGGGGUUGGCGc -3' miRNA: 3'- -UCUCUACCGGUUCACGCCCCAACCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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