Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20408 | 3' | -53.7 | NC_004688.1 | + | 6777 | 0.66 | 0.923718 |
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Target: 5'- -gCCGCaGGccugGGCCuCUUCGGacugGUUCGGGc -3' miRNA: 3'- aaGGCGaCC----UCGG-GAAGCU----UAAGUCC- -5' |
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| 20408 | 3' | -53.7 | NC_004688.1 | + | 61815 | 0.66 | 0.917883 |
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Target: 5'- -gCCGC-GGGGCUCgUCGGAgcugCGGGu -3' miRNA: 3'- aaGGCGaCCUCGGGaAGCUUaa--GUCC- -5' |
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| 20408 | 3' | -53.7 | NC_004688.1 | + | 101075 | 0.66 | 0.91241 |
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Target: 5'- gUCCaaGCUGGAGCCCguaGAGguaccggaggacgucUCGGGc -3' miRNA: 3'- aAGG--CGACCUCGGGaagCUUa--------------AGUCC- -5' |
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| 20408 | 3' | -53.7 | NC_004688.1 | + | 7300 | 0.67 | 0.877506 |
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Target: 5'- cUUCGCUGG-GCCCggaGAAgaugCGGGu -3' miRNA: 3'- aAGGCGACCuCGGGaagCUUaa--GUCC- -5' |
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| 20408 | 3' | -53.7 | NC_004688.1 | + | 84449 | 0.68 | 0.8286 |
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Target: 5'- aUCCgGCgucgGGAGCCag-CG-AUUCAGGa -3' miRNA: 3'- aAGG-CGa---CCUCGGgaaGCuUAAGUCC- -5' |
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| 20408 | 3' | -53.7 | NC_004688.1 | + | 41793 | 0.69 | 0.772718 |
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Target: 5'- -aCCGCguUGGGGUCCUcCGGGaUCGGGc -3' miRNA: 3'- aaGGCG--ACCUCGGGAaGCUUaAGUCC- -5' |
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| 20408 | 3' | -53.7 | NC_004688.1 | + | 2271 | 1.08 | 0.003718 |
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Target: 5'- aUUCCGCUGGAGCCCUUCGAAUUCAGGc -3' miRNA: 3'- -AAGGCGACCUCGGGAAGCUUAAGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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