Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20408 | 5' | -56.8 | NC_004688.1 | + | 43806 | 0.66 | 0.79722 |
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Target: 5'- gUCCCgGCCGGAacGUAgaaCUCGCCGuugagcauggcccGGAu -3' miRNA: 3'- gAGGG-CGGCCU--UAUa--GAGCGGU-------------CCUu -5' |
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| 20408 | 5' | -56.8 | NC_004688.1 | + | 34354 | 0.67 | 0.720276 |
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Target: 5'- aCUCCCgGCUGGccgGUCUCcGCgCGGGAc -3' miRNA: 3'- -GAGGG-CGGCCuuaUAGAG-CG-GUCCUu -5' |
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| 20408 | 5' | -56.8 | NC_004688.1 | + | 91633 | 0.68 | 0.668708 |
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Target: 5'- uUCCgGCCGGAGgggCggaaCGUCGGGAAg -3' miRNA: 3'- gAGGgCGGCCUUauaGa---GCGGUCCUU- -5' |
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| 20408 | 5' | -56.8 | NC_004688.1 | + | 62427 | 0.7 | 0.58502 |
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Target: 5'- -gCCCGCCGGc---UCUUGgCCGGGGAa -3' miRNA: 3'- gaGGGCGGCCuuauAGAGC-GGUCCUU- -5' |
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| 20408 | 5' | -56.8 | NC_004688.1 | + | 3245 | 0.7 | 0.574638 |
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Target: 5'- aCUCCgCGCCagGGAAUAUgguggggacCUCGCCAaGGAu -3' miRNA: 3'- -GAGG-GCGG--CCUUAUA---------GAGCGGU-CCUu -5' |
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| 20408 | 5' | -56.8 | NC_004688.1 | + | 2237 | 1.06 | 0.002408 |
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Target: 5'- gCUCCCGCCGGAAUAUCUCGCCAGGAAg -3' miRNA: 3'- -GAGGGCGGCCUUAUAGAGCGGUCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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