Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20409 | 3' | -54.5 | NC_004688.1 | + | 67594 | 0.66 | 0.891968 |
Target: 5'- -cGUCGGcGGCGGUCGgCGGgcUGAGGUCc -3' miRNA: 3'- aaCGGCCuCCGCUAGU-GCU--GUUCUAG- -5' |
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20409 | 3' | -54.5 | NC_004688.1 | + | 43959 | 0.66 | 0.884859 |
Target: 5'- aUGCUGG-GGCGcAUCggcaugacgGCGGCGGGGUa -3' miRNA: 3'- aACGGCCuCCGC-UAG---------UGCUGUUCUAg -5' |
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20409 | 3' | -54.5 | NC_004688.1 | + | 45182 | 0.66 | 0.875253 |
Target: 5'- aUGCCGGccacgaguucgucgAGGUcGUCGCGcauCAGGGUCu -3' miRNA: 3'- aACGGCC--------------UCCGcUAGUGCu--GUUCUAG- -5' |
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20409 | 3' | -54.5 | NC_004688.1 | + | 31005 | 0.66 | 0.862089 |
Target: 5'- -cGUCGGcguuGGCGAUCACcACGGGGccuUCg -3' miRNA: 3'- aaCGGCCu---CCGCUAGUGcUGUUCU---AG- -5' |
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20409 | 3' | -54.5 | NC_004688.1 | + | 33383 | 0.66 | 0.861294 |
Target: 5'- -cGCCGGAaccuguauccacGGCGAUacucaccCACGGCAcaucgcccgagcGGGUCa -3' miRNA: 3'- aaCGGCCU------------CCGCUA-------GUGCUGU------------UCUAG- -5' |
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20409 | 3' | -54.5 | NC_004688.1 | + | 39701 | 0.67 | 0.845767 |
Target: 5'- cUGuuGGGGGUGGgccgUC-CGGCGGGGUUg -3' miRNA: 3'- aACggCCUCCGCU----AGuGCUGUUCUAG- -5' |
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20409 | 3' | -54.5 | NC_004688.1 | + | 33985 | 0.67 | 0.845767 |
Target: 5'- -cGUCGGGGGCGucaaAUC-CGGguCAGGGUCa -3' miRNA: 3'- aaCGGCCUCCGC----UAGuGCU--GUUCUAG- -5' |
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20409 | 3' | -54.5 | NC_004688.1 | + | 71028 | 0.71 | 0.627077 |
Target: 5'- -cGCuUGGGGGUGAgCGCGcGCAGGAUCu -3' miRNA: 3'- aaCG-GCCUCCGCUaGUGC-UGUUCUAG- -5' |
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20409 | 3' | -54.5 | NC_004688.1 | + | 99856 | 0.71 | 0.595069 |
Target: 5'- cUGCUGGuGGCuggCGCGACcGGAUCg -3' miRNA: 3'- aACGGCCuCCGcuaGUGCUGuUCUAG- -5' |
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20409 | 3' | -54.5 | NC_004688.1 | + | 93829 | 0.71 | 0.595069 |
Target: 5'- -gGCCGGAaaCGAUCGCG-CAGGAUUg -3' miRNA: 3'- aaCGGCCUccGCUAGUGCuGUUCUAG- -5' |
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20409 | 3' | -54.5 | NC_004688.1 | + | 36850 | 0.72 | 0.541374 |
Target: 5'- gUGCCGGuGGUGGUCGCGuucagauACGAGGc- -3' miRNA: 3'- aACGGCCuCCGCUAGUGC-------UGUUCUag -5' |
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20409 | 3' | -54.5 | NC_004688.1 | + | 10661 | 0.76 | 0.345225 |
Target: 5'- -cGCUGGGGGCGGUCagcGCGGCGuuguccgcggccuGGAUCg -3' miRNA: 3'- aaCGGCCUCCGCUAG---UGCUGU-------------UCUAG- -5' |
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20409 | 3' | -54.5 | NC_004688.1 | + | 19607 | 0.77 | 0.307374 |
Target: 5'- aUGCCGG-GGCGAauaCGCGgACAAGGUCu -3' miRNA: 3'- aACGGCCuCCGCUa--GUGC-UGUUCUAG- -5' |
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20409 | 3' | -54.5 | NC_004688.1 | + | 3963 | 1.06 | 0.003508 |
Target: 5'- aUUGCCGGAGGCGAUCACGACAAGAUCc -3' miRNA: 3'- -AACGGCCUCCGCUAGUGCUGUUCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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