Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 20410 | 3' | -55 | NC_004688.1 | + | 36445 | 0.66 | 0.869827 |
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Target: 5'- gCGCCGGACauUCCAaccuGCggGGAC-AGUUCg -3' miRNA: 3'- -GCGGCCUG--AGGU----CGa-CUUGcUCAAGa -5' |
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| 20410 | 3' | -55 | NC_004688.1 | + | 8777 | 0.66 | 0.869068 |
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Target: 5'- uGCCGGACUuggugcgggCCAGCgGggUGcccgcguGGUUCa -3' miRNA: 3'- gCGGCCUGA---------GGUCGaCuuGC-------UCAAGa -5' |
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| 20410 | 3' | -55 | NC_004688.1 | + | 74614 | 0.67 | 0.846103 |
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Target: 5'- gCGCCgauGGACUUCcgccagGGCUGuucuuuGGCGAGUUCg -3' miRNA: 3'- -GCGG---CCUGAGG------UCGAC------UUGCUCAAGa -5' |
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| 20410 | 3' | -55 | NC_004688.1 | + | 55379 | 0.67 | 0.846103 |
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Target: 5'- cCGCCGGGCUC-GGCUGGccgACGAa---- -3' miRNA: 3'- -GCGGCCUGAGgUCGACU---UGCUcaaga -5' |
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| 20410 | 3' | -55 | NC_004688.1 | + | 38550 | 0.69 | 0.755006 |
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Target: 5'- aGCCGGGgUCUucgcuggaaauGCUGAACG-GUUCa -3' miRNA: 3'- gCGGCCUgAGGu----------CGACUUGCuCAAGa -5' |
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| 20410 | 3' | -55 | NC_004688.1 | + | 77402 | 0.71 | 0.631529 |
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Target: 5'- gCGUCGGGgUCgaggGGUUGGGCGAGUUCa -3' miRNA: 3'- -GCGGCCUgAGg---UCGACUUGCUCAAGa -5' |
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| 20410 | 3' | -55 | NC_004688.1 | + | 82557 | 0.72 | 0.568791 |
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Target: 5'- gCGCCG----CCAGCUGGACGAGUUUc -3' miRNA: 3'- -GCGGCcugaGGUCGACUUGCUCAAGa -5' |
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| 20410 | 3' | -55 | NC_004688.1 | + | 24471 | 0.76 | 0.378191 |
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Target: 5'- uGCCGuACUCCAGCUGGG-GGGUUCc -3' miRNA: 3'- gCGGCcUGAGGUCGACUUgCUCAAGa -5' |
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| 20410 | 3' | -55 | NC_004688.1 | + | 4690 | 1.07 | 0.003295 |
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Target: 5'- gCGCCGGACUCCAGCUGAACGAGUUCUu -3' miRNA: 3'- -GCGGCCUGAGGUCGACUUGCUCAAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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