Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20410 | 5' | -52.9 | NC_004688.1 | + | 84134 | 0.66 | 0.948109 |
Target: 5'- gGAGAcauccacGCC-CGUGUAGGCGaugGAcUCCa -3' miRNA: 3'- aCUCU-------CGGaGCACGUCCGUg--UUaAGG- -5' |
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20410 | 5' | -52.9 | NC_004688.1 | + | 9071 | 0.67 | 0.923012 |
Target: 5'- cGAGAGCCUCcggaagggaGcGCAGGC-CGAcgCUg -3' miRNA: 3'- aCUCUCGGAG---------CaCGUCCGuGUUaaGG- -5' |
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20410 | 5' | -52.9 | NC_004688.1 | + | 2853 | 0.67 | 0.923012 |
Target: 5'- -cGGGGCC-C-UGCAGGCGCAaucgagGUUCUc -3' miRNA: 3'- acUCUCGGaGcACGUCCGUGU------UAAGG- -5' |
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20410 | 5' | -52.9 | NC_004688.1 | + | 799 | 0.67 | 0.917115 |
Target: 5'- cGGGAGaCCUCGUGaucacccuggAGGaCGCcAUUCCc -3' miRNA: 3'- aCUCUC-GGAGCACg---------UCC-GUGuUAAGG- -5' |
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20410 | 5' | -52.9 | NC_004688.1 | + | 86235 | 0.67 | 0.907135 |
Target: 5'- cGGGAGCUcgUCGagggGCAGGCccaGCAcgccugccgcauacgAUUCCa -3' miRNA: 3'- aCUCUCGG--AGCa---CGUCCG---UGU---------------UAAGG- -5' |
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20410 | 5' | -52.9 | NC_004688.1 | + | 12902 | 0.68 | 0.852611 |
Target: 5'- cGAGaAGUCUCGgccggGCAGGUGCucUUCa -3' miRNA: 3'- aCUC-UCGGAGCa----CGUCCGUGuuAAGg -5' |
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20410 | 5' | -52.9 | NC_004688.1 | + | 95550 | 0.69 | 0.826929 |
Target: 5'- gGAGcGGCCUCcgaaGCuuGGGCugGGUUCCu -3' miRNA: 3'- aCUC-UCGGAGca--CG--UCCGugUUAAGG- -5' |
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20410 | 5' | -52.9 | NC_004688.1 | + | 7623 | 0.7 | 0.780354 |
Target: 5'- ----cGCCUCGUcGCGGGCACuGAUguagCCg -3' miRNA: 3'- acucuCGGAGCA-CGUCCGUG-UUAa---GG- -5' |
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20410 | 5' | -52.9 | NC_004688.1 | + | 9682 | 0.73 | 0.602501 |
Target: 5'- cGAGAGCCUCGcGaAGGCugAug-CCg -3' miRNA: 3'- aCUCUCGGAGCaCgUCCGugUuaaGG- -5' |
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20410 | 5' | -52.9 | NC_004688.1 | + | 75983 | 0.75 | 0.528569 |
Target: 5'- --uGAGCCUCGUcGCGGGuCGCA--UCCu -3' miRNA: 3'- acuCUCGGAGCA-CGUCC-GUGUuaAGG- -5' |
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20410 | 5' | -52.9 | NC_004688.1 | + | 4654 | 1.12 | 0.002182 |
Target: 5'- uUGAGAGCCUCGUGCAGGCACAAUUCCu -3' miRNA: 3'- -ACUCUCGGAGCACGUCCGUGUUAAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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