Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
20411 | 5' | -53.9 | NC_004688.1 | + | 101581 | 0.66 | 0.935753 |
Target: 5'- gGGGAgcaGGUCCacacgGCCGaUCUGGg -3' miRNA: 3'- gUCCUag-CUAGGgaa--CGGCaAGACCg -5' |
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20411 | 5' | -53.9 | NC_004688.1 | + | 91049 | 0.66 | 0.925096 |
Target: 5'- gAGG--UGAUCcaCCUUGCCGgUCUGGa -3' miRNA: 3'- gUCCuaGCUAG--GGAACGGCaAGACCg -5' |
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20411 | 5' | -53.9 | NC_004688.1 | + | 101394 | 0.66 | 0.925096 |
Target: 5'- aGGGAUCGGUgcggCCCUacGCUcugCUGGCu -3' miRNA: 3'- gUCCUAGCUA----GGGAa-CGGcaaGACCG- -5' |
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20411 | 5' | -53.9 | NC_004688.1 | + | 65544 | 0.66 | 0.919384 |
Target: 5'- gCAGGGUgucaaagcCGAUCCa--GCCGUUCUcGGa -3' miRNA: 3'- -GUCCUA--------GCUAGGgaaCGGCAAGA-CCg -5' |
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20411 | 5' | -53.9 | NC_004688.1 | + | 31456 | 0.66 | 0.919384 |
Target: 5'- cCAGGAgcugcucgUgGAUgCC-UGCUGUUCUGcGCg -3' miRNA: 3'- -GUCCU--------AgCUAgGGaACGGCAAGAC-CG- -5' |
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20411 | 5' | -53.9 | NC_004688.1 | + | 7041 | 0.66 | 0.913418 |
Target: 5'- cCAGcuGUCGAUCUCUUGuuGaUCgGGCc -3' miRNA: 3'- -GUCc-UAGCUAGGGAACggCaAGaCCG- -5' |
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20411 | 5' | -53.9 | NC_004688.1 | + | 63686 | 0.67 | 0.898068 |
Target: 5'- -cGGAUCaacgccucaguuUCCCUUGCggCGUUCUuGGCg -3' miRNA: 3'- guCCUAGcu----------AGGGAACG--GCAAGA-CCG- -5' |
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20411 | 5' | -53.9 | NC_004688.1 | + | 58932 | 0.67 | 0.879836 |
Target: 5'- -uGGAUgaccUGGUCCCUgcGCUGggagCUGGCg -3' miRNA: 3'- guCCUA----GCUAGGGAa-CGGCaa--GACCG- -5' |
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20411 | 5' | -53.9 | NC_004688.1 | + | 48902 | 0.68 | 0.864723 |
Target: 5'- uGGGuucgCGGUCCCUgUGCCGcgg-GGCu -3' miRNA: 3'- gUCCua--GCUAGGGA-ACGGCaagaCCG- -5' |
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20411 | 5' | -53.9 | NC_004688.1 | + | 84426 | 0.69 | 0.795914 |
Target: 5'- uCAGGaAUCGcUCCaggcgGCCcUUCUGGCg -3' miRNA: 3'- -GUCC-UAGCuAGGgaa--CGGcAAGACCG- -5' |
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20411 | 5' | -53.9 | NC_004688.1 | + | 25706 | 0.71 | 0.706749 |
Target: 5'- uGGGGUCGAgUCCCgauucGCCGa--UGGCg -3' miRNA: 3'- gUCCUAGCU-AGGGaa---CGGCaagACCG- -5' |
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20411 | 5' | -53.9 | NC_004688.1 | + | 11172 | 0.71 | 0.664922 |
Target: 5'- -cGGGUCGGUCacgaCCggcagGCCGUgcaUCUGGCc -3' miRNA: 3'- guCCUAGCUAG----GGaa---CGGCA---AGACCG- -5' |
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20411 | 5' | -53.9 | NC_004688.1 | + | 75971 | 0.74 | 0.51854 |
Target: 5'- -cGGGUCGcAUCCUUgaaGCCGUUCUuGGUg -3' miRNA: 3'- guCCUAGC-UAGGGAa--CGGCAAGA-CCG- -5' |
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20411 | 5' | -53.9 | NC_004688.1 | + | 4764 | 1.12 | 0.001894 |
Target: 5'- cCAGGAUCGAUCCCUUGCCGUUCUGGCg -3' miRNA: 3'- -GUCCUAGCUAGGGAACGGCAAGACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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